Anti-AIMP2/p38 antibody (ab125218)
Key features and details
- Rabbit polyclonal to AIMP2/p38
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-AIMP2/p38 antibody
See all AIMP2/p38 primary antibodies -
Description
Rabbit polyclonal to AIMP2/p38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide from internal sequence amino acids of Human AIMP2/p38.
-
Positive control
- Rat liver, lung, kidney, and brain lysates; Jurkat, CEM, HUT and U93T whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. The detection limit for ab125218 is 1ng/lane under non-reducing and reducing conditions.
|
|
IHC-P |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. The detection limit for ab125218 is 1ng/lane under non-reducing and reducing conditions. |
IHC-P
Use a concentration of 1 µg/ml. |
Target
-
Function
Required for assembly and stability of the aminoacyl-tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. -
Sequence similarities
Contains 1 GST C-terminal domain. -
Post-translational
modificationsPhosphorylated on serine residues in response to UV irradiation.
Ubiquitinated by PARK2, leading to its degradation by the proteasome. Mutant PARK2 fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7965 Human
- Entrez Gene: 231872 Mouse
- Entrez Gene: 288480 Rat
- Omim: 600859 Human
- SwissProt: Q13155 Human
- SwissProt: Q8R010 Mouse
- SwissProt: Q32PX2 Rat
- Unigene: 301613 Human
see all -
Alternative names
- Aimp2 antibody
- AIMP2_HUMAN antibody
- Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 antibody
see all
Images
-
All lanes : Anti-AIMP2/p38 antibody (ab125218) at 1 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rabbit IgG (55kDa)
Lane 6 : Jurkat whole cell lysate
Lane 7 : CEM whole cell lysate
Lane 8 : HUT whole cell lysate
Lane 9 : U93T whole cell lysate
Predicted band size: 35 kDa -
ab125218 at 1ug/ml in paraffin embedded Human tumour tissue by immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab125218 has not yet been referenced specifically in any publications.