Key features and details
- Rabbit polyclonal to AIMP2/p38
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-AIMP2/p38 antibody
See all AIMP2/p38 primary antibodies
DescriptionRabbit polyclonal to AIMP2/p38
Specificityab180897 is predicted to not cross-react with EMAP II.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human AIMP2/p38 (internal sequence). (16 amino acid peptide; NP_006294).
Database link: Q13155
- HeLa cell lysate; Rat small intestine tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionRequired for assembly and stability of the aminoacyl-tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.
Sequence similaritiesContains 1 GST C-terminal domain.
modificationsPhosphorylated on serine residues in response to UV irradiation.
Ubiquitinated by PARK2, leading to its degradation by the proteasome. Mutant PARK2 fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease.
Cellular localizationCytoplasm > cytosol. Nucleus. Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus.
- Information by UniProt
- Aimp2 antibody
- AIMP2_HUMAN antibody
- Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 antibody
All lanes : Anti-AIMP2/p38 antibody (ab180897) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue labeling AIMP2/p38 with ab180897 at 5 μg/ml.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue labeling AIMP2/p38 with ab180897 at 20 µg/ml.
ab180897 has not yet been referenced specifically in any publications.