Overview

  • Product name

  • Description

    Rabbit polyclonal to AIMP2/p38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human AIMP2/p38 (internal sequence). The exact sequence is proprietary.
    Database link: Q13155

  • Positive control

    • WB: HL-60 whole cell lysate. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for assembly and stability of the aminoacyl-tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.
  • Sequence similarities

    Contains 1 GST C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues in response to UV irradiation.
    Ubiquitinated by PARK2, leading to its degradation by the proteasome. Mutant PARK2 fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aimp2 antibody
    • AIMP2_HUMAN antibody
    • Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 antibody
    • Aminoacyl tRNA synthetase complex interacting multifunctional protein 2 antibody
    • ARS interacting multi functional protein 2 antibody
    • JTV 1 antibody
    • JTV 1 protein antibody
    • JTV1 antibody
    • JTV1 gene antibody
    • Multisynthase complex auxiliary component p38 antibody
    • Multisynthetase complex auxiliary component p38 antibody
    • P38 antibody
    • PRO0992 antibody
    • Protein JTV 1 antibody
    • Protein JTV-1 antibody
    • tRNA SYNTHETASE COFACTOR p38 antibody
    see all

Images

  • Anti-AIMP2/p38 antibody (ab228004) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa



    10% SDS-PAGE

  • Paraffin-embedded human kidney tissue stained for AIMP2/p38 with ab228004 at 1/500 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AIMP2/p38 (green) using ab228004 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

References

This product has been referenced in:

See 1 Publication for this product

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