Overview

  • Product name

  • Description

    Rabbit polyclonal to AIMP3/p18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-174 of Human AIMP3/p18 (UniProt: O43324).

  • Positive control

    • A431, Raji and Mouse brain whole cell lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Positive modulator of ATM response to DNA damage.
  • Tissue specificity

    Down-regulated in various cancer tissues.
  • Sequence similarities

    Contains 1 GST C-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • AIMP 3 antibody
    • AIMP3 antibody
    • Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 antibody
    • ARS interacting multifunctional protein 3 antibody
    • EEF1E1 antibody
    • Elongation factor p18 antibody
    • Eukaryotic translation elongation factor 1 epsilon 1 antibody
    • Eukaryotic translation elongation factor 1 epsilon-1 antibody
    • Homolog of rat elongation factor p18 antibody
    • MCA3_HUMAN antibody
    • Multisynthase complex auxiliary component p18 antibody
    • Multisynthetase complex auxiliary component p18 antibody
    • p18 antibody
    • p18 component of aminoacyl tRNA synthetase complex antibody
    see all

Images

  • All lanes : Anti-AIMP3/p18 antibody (ab155689) at 1/500 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : AIMP3/p18 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa

  • Anti-AIMP3/p18 antibody (ab155689) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 19 kDa



    12% SDS PAGE
  • Anti-AIMP3/p18 antibody (ab155689) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 19 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling AIMP3/p18 with ab155689 at 1/200 dilution. Lower image is merged with DNA probe.

References

ab155689 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155689.
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