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  1. Link

    aip-antibody-epr13585-ab192024.pdf

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Neuroscience Neurotransmission Receptors / Channels More Ion Channels
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-AIP antibody [EPR13585] (ab192024)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-AIP antibody [EPR13585] (ab192024)
  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
  • Anti-AIP antibody [EPR13585] (ab192024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13585] to AIP
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human AIP protein (ab109934)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human AIP knockout HeLa cell line (ab265502)

View more associated products

Overview

  • Product name

    Anti-AIP antibody [EPR13585]
    See all AIP primary antibodies
  • Description

    Rabbit monoclonal [EPR13585] to AIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HepG2, C6, Raw264.7 and NIH/3T3 cell lysates; Human thymus, Mouse brain, Mouse heart, Mouse kidney, Mouse spleen, Rat heart and Rat kidney lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13585
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels

Associated products

  • Alternative Versions

    • Anti-AIP antibody [EPR13585] - BSA and Azide free (ab251106)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human AIP knockout HeLa cell line (ab265502)
  • KO cell lysates

    • Human AIP knockout HeLa cell lysate (ab257822)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human AIP protein (ab109934)
  • Related Products

    • Recombinant Human AIP protein (ab109934)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.
    Cellular negative regulator of the hepatitis B virus (HBV) X protein.
  • Tissue specificity

    Widely expressed. Higher levels seen in the heart, placenta and skeletal muscle. Not expressed in the liver.
  • Involvement in disease

    Defects in AIP are a cause of familial isolated pituitary adenoma (FIPA) [MIM:102200].
    Defects in AIP are a cause of growth hormone-secreting pituitary adenoma (GHSPA) [MIM:102200]; also known as familial isolated somatotropinomas (FIS) or isolated familial somatotropinoma (IFS) or familial somatotrophinoma or acromegaly due to pituitary adenoma.
    Defects in AIP are a cause of ACTH-secreting pituitary adenoma (ASPA) [MIM:219090]; also known as pituitary Cushing disease. A pituary adenoma resulting in excessive production of adrenocorticotropic hormone. This leads to hypersecretion of cortisol by the adrenal glands and ACTH-dependent Cushing syndrome. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
    Defects in AIP are a cause of prolactin-secreting pituitary adenoma (PSPA) [MIM:600634]; also known as prolactinoma. Prolactin-secreting pituitary adenoma is the most common type of hormonally active pituitary adenoma.
  • Sequence similarities

    Contains 1 PPIase FKBP-type domain.
    Contains 2 TPR repeats.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O00170 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9049 Human
    • Entrez Gene: 11632 Mouse
    • Entrez Gene: 282827 Rat
    • Omim: 605555 Human
    • SwissProt: O00170 Human
    • SwissProt: O08915 Mouse
    • SwissProt: Q5FWY5 Rat
    • Unigene: 412433 Human
    • Unigene: 10433 Mouse
    • Unigene: 95160 Rat
    see all
  • Alternative names

    • AH receptor interacting protein antibody
    • AH receptor-interacting protein antibody
    • AIP antibody
    • AIP_HUMAN antibody
    • ARA 9 antibody
    • ARA9 antibody
    • Aryl hydrocarbon receptor interacting protein antibody
    • Aryl-hydrocarbon receptor-interacting protein antibody
    • fa03h10 antibody
    • FKBP 16 antibody
    • FKBP 37 antibody
    • FKBP16 antibody
    • FKBP37 antibody
    • HBV X associated protein 2 antibody
    • HBV X associated protein antibody
    • HBV X-associated protein 2 antibody
    • HBVX associated protein antibody
    • Immunophilin homolog ARA 9 antibody
    • Immunophilin homolog ARA9 antibody
    • SMTPHN antibody
    • XAP 2 antibody
    • XAP-2 antibody
    • XAP2 antibody
    see all

Images

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : AIP knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human thymus cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : C6 cell lysate
    Lane 8 : Raw 264.7 cell lysate
    Lane 9 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM /TBST.

  • Anti-AIP antibody [EPR13585] (ab192024)
    Anti-AIP antibody [EPR13585] (ab192024)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab192024? Please let us know so that we can cite the reference in this datasheet.

ab192024 has been referenced in 1 publication.

  • Cai F  et al. A Novel Mutation of Aryl Hydrocarbon Receptor Interacting Protein Gene Associated with Familial Isolated Pituitary Adenoma Mediates Tumor Invasion and Growth Hormone Hypersecretion. World Neurosurg N/A:N/A (2018). PubMed: 30447469

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