Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-AIP antibody [EPR13585] (ab192024)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13585] to AIP
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AIP antibody [EPR13585]
    See all AIP primary antibodies

  • Description

    Rabbit monoclonal [EPR13585] to AIP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human AIP aa 1-100. The exact sequence is proprietary.
    Database link: O00170

  • Positive control

    • WB: HeLa, Jurkat, HepG2, C6, Raw264.7 and NIH/3T3 cell lysates; Human thymus, Mouse brain, Mouse heart, Mouse kidney, Mouse spleen, Rat heart and Rat kidney lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13585

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.
    Cellular negative regulator of the hepatitis B virus (HBV) X protein.

  • Tissue specificity

    Widely expressed. Higher levels seen in the heart, placenta and skeletal muscle. Not expressed in the liver.

  • Involvement in disease

    Defects in AIP are a cause of familial isolated pituitary adenoma (FIPA) [MIM:102200].
    Defects in AIP are a cause of growth hormone-secreting pituitary adenoma (GHSPA) [MIM:102200]; also known as familial isolated somatotropinomas (FIS) or isolated familial somatotropinoma (IFS) or familial somatotrophinoma or acromegaly due to pituitary adenoma.
    Defects in AIP are a cause of ACTH-secreting pituitary adenoma (ASPA) [MIM:219090]; also known as pituitary Cushing disease. A pituary adenoma resulting in excessive production of adrenocorticotropic hormone. This leads to hypersecretion of cortisol by the adrenal glands and ACTH-dependent Cushing syndrome. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
    Defects in AIP are a cause of prolactin-secreting pituitary adenoma (PSPA) [MIM:600634]; also known as prolactinoma. Prolactin-secreting pituitary adenoma is the most common type of hormonally active pituitary adenoma.

  • Sequence similarities

    Contains 1 PPIase FKBP-type domain.
    Contains 2 TPR repeats.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AH receptor interacting protein antibody
    • AH receptor-interacting protein antibody
    • AIP antibody
    • AIP_HUMAN antibody
    • ARA 9 antibody
    • ARA9 antibody
    • Aryl hydrocarbon receptor interacting protein antibody
    • Aryl-hydrocarbon receptor-interacting protein antibody
    • fa03h10 antibody
    • FKBP 16 antibody
    • FKBP 37 antibody
    • FKBP16 antibody
    • FKBP37 antibody
    • HBV X associated protein 2 antibody
    • HBV X associated protein antibody
    • HBV X-associated protein 2 antibody
    • HBVX associated protein antibody
    • Immunophilin homolog ARA 9 antibody
    • Immunophilin homolog ARA9 antibody
    • SMTPHN antibody
    • XAP 2 antibody
    • XAP-2 antibody
    • XAP2 antibody
    see all

Images

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : AIP knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human thymus cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : C6 cell lysate
    Lane 8 : Raw 264.7 cell lysate
    Lane 9 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM /TBST.

  • Anti-AIP antibody [EPR13585] (ab192024)
    Anti-AIP antibody [EPR13585] (ab192024)

References (1)

Publishing research using ab192024? Please let us know so that we can cite the reference in this datasheet.

ab192024 has been referenced in 1 publication.

  • Cai F  et al. A Novel Mutation of Aryl Hydrocarbon Receptor Interacting Protein Gene Associated with Familial Isolated Pituitary Adenoma Mediates Tumor Invasion and Growth Hormone Hypersecretion. World Neurosurg N/A:N/A (2018). PubMed: 30447469

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