Recombinant Anti-AIP antibody [EPR13585] - BSA and Azide free (ab251106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13585] to AIP - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AIP antibody [EPR13585] - BSA and Azide free
See all AIP primary antibodies -
Description
Rabbit monoclonal [EPR13585] to AIP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HepG2 cell lysates.
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General notes
ab251106 is the carrier-free version of ab192024.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR13585 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- NIH/3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Jurkat whole cell lysate (ab7899)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.
Cellular negative regulator of the hepatitis B virus (HBV) X protein. -
Tissue specificity
Widely expressed. Higher levels seen in the heart, placenta and skeletal muscle. Not expressed in the liver. -
Involvement in disease
Defects in AIP are a cause of familial isolated pituitary adenoma (FIPA) [MIM:102200].
Defects in AIP are a cause of growth hormone-secreting pituitary adenoma (GHSPA) [MIM:102200]; also known as familial isolated somatotropinomas (FIS) or isolated familial somatotropinoma (IFS) or familial somatotrophinoma or acromegaly due to pituitary adenoma.
Defects in AIP are a cause of ACTH-secreting pituitary adenoma (ASPA) [MIM:219090]; also known as pituitary Cushing disease. A pituary adenoma resulting in excessive production of adrenocorticotropic hormone. This leads to hypersecretion of cortisol by the adrenal glands and ACTH-dependent Cushing syndrome. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
Defects in AIP are a cause of prolactin-secreting pituitary adenoma (PSPA) [MIM:600634]; also known as prolactinoma. Prolactin-secreting pituitary adenoma is the most common type of hormonally active pituitary adenoma. -
Sequence similarities
Contains 1 PPIase FKBP-type domain.
Contains 2 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9049 Human
- Entrez Gene: 11632 Mouse
- Entrez Gene: 282827 Rat
- Omim: 605555 Human
- SwissProt: O00170 Human
- SwissProt: O08915 Mouse
- SwissProt: Q5FWY5 Rat
- Unigene: 412433 Human
see all -
Alternative names
- AH receptor interacting protein antibody
- AH receptor-interacting protein antibody
- AIP antibody
see all
Images
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All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : AIP knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab192024).
Lanes 1-3: Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.
ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251106 has not yet been referenced specifically in any publications.