Recombinant Anti-AIPL1 antibody [EPR7711] (ab128960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7711] to AIPL1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AIPL1 antibody [EPR7711]
See all AIPL1 primary antibodies -
Description
Rabbit monoclonal [EPR7711] to AIPL1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AIPL1 aa 350-450. The exact sequence is proprietary.
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Positive control
- Y79 cell lysate; permeabilized Y79 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.25 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7711 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
Target
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Function
May be important in protein trafficking and/or protein folding and stabilization. -
Tissue specificity
Highly expressed in retina. Specifically localized to the developing photoreceptor layer and within the photoreceptors of the adult retina. -
Involvement in disease
Defects in AIPL1 are the cause of Leber congenital amaurosis type 4 (LCA4) [MIM:604393]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. -
Sequence similarities
Contains 1 PPIase FKBP-type domain.
Contains 3 TPR repeats. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23746 Human
- Omim: 604392 Human
- SwissProt: Q9NZN9 Human
- Unigene: 279887 Human
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Alternative names
- A930007I01Rik antibody
- Aipl1 antibody
- AIPL1_HUMAN antibody
see all
Images
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ab128960, at 1/10 dilution staining AIPL1 (red) in permeabilized Y79 cells, compared with a negative rabbit IgG (green) by intracellular flow cytometry.
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Anti-AIPL1 antibody [EPR7711] (ab128960) at 1/10000 dilution + Y79 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128960 has not yet been referenced specifically in any publications.