Product nameAnti-AJM1 antibody
DescriptionRabbit polyclonal to AJM1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey, Gorilla
- Hman bone marrow tissue; RT4, U251 MG cell lysates, Human liver tissue lysate
This product was previously labelled as C9orf172
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- C9orf172 antibody
- chromosome 9 open reading frame 172 antibody
- CI172_HUMAN antibody
- Uncharacterized protein C9orf172 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and focal adhesions. Recommended concentration of ab121654 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-AJM1 antibody (ab121654) at 1/500 dilution
Lane 1 : RT4 cell lysates
Lane 2 : U251 MG cell lysates
Lane 3 : Human Plasma
Lane 4 : Liver tissue lysate
Lane 5 : Tonsil tissue lysate
ab121654, at 1/150 dilution, staining AJM1 in Human bone marrow tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
ab121654 has not yet been referenced specifically in any publications.