Key features and details
- Rabbit polyclonal to Ajuba
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ajuba antibody
See all Ajuba primary antibodies
DescriptionRabbit polyclonal to Ajuba
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- IHC-P: Human esophagus, fallopian tube, kidney, skin and placenta tissue. WB: HepG2 cell lysate; Ajuba overexpression HEK-293T lysate. ICC/IF: U-251 MG cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceAjuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
Cellular localizationCytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
- Ajuba LIM protein antibody
- Ajuba, mouse, homolog of antibody
- Jub ajuba homolog antibody
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Ajuba (green) using ab244285 at 4 µg/ml in ICC/IF.
Anti-Ajuba antibody (ab244285) at 0.4 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 57 kDa
Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemical staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244285 has not yet been referenced specifically in any publications.