Key features and details
- Rabbit polyclonal to Ajuba
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ajuba antibody
See all Ajuba primary antibodies
DescriptionRabbit polyclonal to Ajuba
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Ajuba aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
RelevanceAjuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
Cellular localizationCytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
- Ajuba LIM protein antibody
- Ajuba, mouse, homolog of antibody
- Jub ajuba homolog antibody
All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 3 :
WI-38 whole cell lysate (ab3960)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 23 kDa, 48 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab64451 has been referenced in 2 publications.
- Fowler S et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). PubMed: 29934626
- Kalan S et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . PubMed: 23755068