Overview

  • Product name

  • Description

    Rabbit polyclonal to Ajuba
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Ajuba aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93791)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.

Properties

Applications

Our Abpromise guarantee covers the use of ab64451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Relevance

    Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
  • Cellular localization

    Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
  • Database links

  • Alternative names

    • Ajuba LIM protein antibody
    • Ajuba, mouse, homolog of antibody
    • Jub ajuba homolog antibody
    • Jub antibody
    • LIM domain containing protein ajuba antibody
    • MGC15563 antibody
    • Protein ajuba antibody
    see all

Images

  • All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 3 : WI-38 whole cell lysate (ab3960)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 23 kDa, 48 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes

References

This product has been referenced in:

  • Fowler S  et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). Read more (PubMed: 29934626) »
  • Kalan S  et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . Read more (PubMed: 23755068) »
See all 2 Publications for this product

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