Key features and details
- Rabbit polyclonal to AK 1
- Suitable for: WB, IHC-P
- Reacts with: Hamster, Human
- Isotype: IgG
Product nameAnti-AK 1 antibody
See all AK 1 primary antibodies
DescriptionRabbit polyclonal to AK 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Hamster, Human
Synthetic peptide corresponding to Human AK 1 (C terminal) conjugated to keyhole limpet haemocyanin.
Previously labelled as Adenylate Kinase 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesab71621 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab71621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Involvement in diseaseDefects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
Sequence similaritiesBelongs to the adenylate kinase family.
- Information by UniProt
- Adenylate kinase 1 antibody
- Adenylate kinase isoenzyme 1 antibody
- Adenylate kinase soluble antibody
Anti-AK 1 antibody (ab71621) at 1/100 dilution + CHO cell lysate at 12.5 µg
Predicted band size: 22 kDa
Observed band size: 22 kDa
Multiple additional bands are observed. We are unsure as to the identity of these extra bands.
ab71621 at 1/50 dilution, staining AK 1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
ab71621 has not yet been referenced specifically in any publications.