Overview

  • Product name

  • Description

    Rabbit polyclonal to AK 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Human AK 1 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Human hepatocarcinoma tissue. CHO cell lysate.
  • General notes

    Previously labelled as Adenylate Kinase 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab71621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
  • Involvement in disease

    Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
  • Sequence similarities

    Belongs to the adenylate kinase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenylate kinase 1 antibody
    • Adenylate kinase isoenzyme 1 antibody
    • Adenylate kinase soluble antibody
    • AK 1 antibody
    • Ak1 antibody
    • ATP AMP transphosphorylase antibody
    • ATP-AMP transphosphorylase 1 antibody
    • KAD1 antibody
    • KAD1_HUMAN antibody
    • Myokinase antibody
    see all

Images

  • Anti-AK 1 antibody (ab71621) at 1/100 dilution + CHO cell lysate at 12.5 µg

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Multiple additional bands are observed. We are unsure as to the identity of these extra bands.
  • ab71621 at 1/50 dilution, staining AK 1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References

ab71621 has not yet been referenced specifically in any publications.

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