Overview

  • Product name
    AK1 Activity Assay Kit (Colorimetric/Fluorometric)
  • Detection method
    Colorimetric/Fluorometric
  • Sample type
    Cell Lysate, Purified protein, Tissue Lysate
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    AK1 Activity Assay Kit (Colorimetric/Fluorometric) (ab211095) provides a simple, sensitive and reliable method to detect AK1 activity in cell and tissue lysates, mitochondrial lysates or purified protein sample. In this assay, ATP generated by AK1 from ADP can be detected through a multi-step reaction, via the generation of an intermediate that reacts with a probe to form an end product that can be easily quantified colorimetrically (OD = 570 nm) or fluorometrically (Ex/Em = 535/587 nm).


    See Adenylate Kinase Cytotoxicity Assay Kit ab228557 for a simple luminometric assay to measure adenylate kinase in cell culture media. 


     

  • Notes

    AK1 (AK, ADK, myokinase, EC 2.7.4.3) is a phosphotransferase that catalyzes the interconversion of two molecules of ADP to generate ATP and AMP. AK1 is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. Nine isoforms of AK1 have been identified in mammals, differing in molecular weight, tissue distribution, subcellular localization, substrate and phosphate donor specificity, and kinetic properties. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. AK1 also plays an important role in post-ischemic recovery and in apoptosis.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    ADP Substrate 1 x 200µl
    AK1 Assay Buffer 1 x 25ml
    AK1 Convertor (5 U) 1 vial
    AK1 Developer (50 U) 1 vial
    AK1 Probe 1 x 200µl
    ATP Standard (1 μmol) 1 vial
    Positive Control (AK Enzyme) (10 mU) 1 vial
  • Research areas
  • Function
    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
  • Involvement in disease
    Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
  • Sequence similarities
    Belongs to the adenylate kinase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • Adenylate kinase 1
    • Adenylate kinase isoenzyme 1
    • Adenylate kinase soluble
    • AK 1
    • Ak1
    • ATP AMP transphosphorylase
    • ATP-AMP transphosphorylase 1
    • KAD1
    • KAD1_HUMAN
    • Myokinase
    see all

Images

  • Kinetic curves measured at OD 570 nm (colorimetric detection) showing AK1 activity detection in positive control (5µL) and bovine heart mitochondrial lysate (500 ng). Blank: assay buffer.

  • Kinetic curves measured at Ex/Em = 535/587 nm (fluorometric detection) showing AK1 activity detection in positive control (4µL) and bovine heart mitochondrial lysate (50 ng). Blank: assay buffer.

  • Typical ATP Standard calibration curve using colorimetric reading.

Protocols

References

ab211095 has not yet been referenced specifically in any publications.

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