Overview

  • Product name

  • Description

    Rabbit polyclonal to AK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human AK2 (internal sequence). The exact sequence is proprietary.
    Database link: P54819

  • Positive control

    • WB: NCI-H1299 and HepG2 whole cell lysates. IHC-P: Rat liver tissue; NCIN87 xenograft tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
  • Tissue specificity

    Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
  • Involvement in disease

    Defects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
  • Sequence similarities

    Belongs to the adenylate kinase family. AK2 subfamily.
  • Cellular localization

    Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenylate kinase 2 antibody
    • adenylate kinase 2, mitochondrial antibody
    • Adenylate kinase isoenzyme 2 antibody
    • ADK2 antibody
    • AK 2 antibody
    • ak2 antibody
    • ATP AMP transphosphorylase antibody
    • ATP-AMP transphosphorylase 2 antibody
    • EC 2.7.4.3 antibody
    • KAD2_HUMAN antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-AK2 antibody (ab228786) at 1/1000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : AK2 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa

  • All lanes : Anti-AK2 antibody (ab228786) at 1/1000 dilution

    Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded rat liver tissue stained for AK2 using ab228786 at 1/500 dilution in immunohistochemical analysis.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) stained for AK2 (green) using ab228786 at 1/200 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded NCIN87 xenograft stained for AK2 using ab228786 at 1/500 dilution in immunohistochemical analysis.

References

ab228786 has not yet been referenced specifically in any publications.

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