Recombinant
RabMAb

Anti-AK2 antibody [EPR11387(B)] (ab157206)

Overview

  • Product name
    Anti-AK2 antibody [EPR11387(B)]
    See all AK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11387(B)] to AK2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human AK2 (Uniprot: P54819)

  • Positive control
    • HepG2 and Hela cell lysates; fetal kidney, fetal heart and fetal liver lysates; heart and liver tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
    • Tissue specificity
      Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
    • Involvement in disease
      Defects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
    • Sequence similarities
      Belongs to the adenylate kinase family. AK2 subfamily.
    • Cellular localization
      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenylate kinase 2 antibody
      • adenylate kinase 2, mitochondrial antibody
      • Adenylate kinase isoenzyme 2 antibody
      • ADK2 antibody
      • AK 2 antibody
      • ak2 antibody
      • ATP AMP transphosphorylase antibody
      • ATP-AMP transphosphorylase 2 antibody
      • EC 2.7.4.3 antibody
      • KAD2_HUMAN antibody
      • mitochondrial antibody
      see all

    Images

    • All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : fetal kidney lysate
      Lane 3 : fetal heart lysate
      Lane 4 : HeLa lysate
      Lane 5 : fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling AK2 using ab157206 at a 1/50 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling AK2 using ab157206 at a 1/50 dilution.
    • Flow cytometry analysis of permeabilized HeLa cells labeling AK2 (red), using ab157206 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157206 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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