Product nameAnti-AK2 antibody [EPR11387(B)]
See all AK2 primary antibodies
DescriptionRabbit monoclonal [EPR11387(B)] to AK2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human AK2 (Uniprot: P54819)
- Recombinant Human AK2 protein (ab78832) can be used as a positive control in WB. HepG2 and Hela cell lysates; fetal kidney, fetal heart and fetal liver lysates; heart and liver tissues; HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab157206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
Tissue specificityPresent in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
Involvement in diseaseDefects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
Sequence similaritiesBelongs to the adenylate kinase family. AK2 subfamily.
Cellular localizationMitochondrion intermembrane space.
- Information by UniProt
- Adenylate kinase 2 antibody
- adenylate kinase 2, mitochondrial antibody
- Adenylate kinase isoenzyme 2 antibody
All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : fetal kidney lysate
Lane 3 : fetal heart lysate
Lane 4 : HeLa lysate
Lane 5 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling AK2 using ab157206 at a 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling AK2 using ab157206 at a 1/50 dilution.
Flow cytometry analysis of permeabilized HeLa cells labeling AK2 (red), using ab157206 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
ab157206 has not yet been referenced specifically in any publications.