Overview

  • Product name

    Anti-AK3L1 antibody [EPR7678]
    See all AK3L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7678] to AK3L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human AK3L1. (P27144)

  • Positive control

    • MCF-7, HepG2, and Raji cell lysates; Human liver tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP.
    • Sequence similarities

      Belongs to the adenylate kinase family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adenylate kinase 3 like 1 antibody
      • Adenylate kinase 3-like antibody
      • adenylate kinase 4 antibody
      • Adenylate kinase isoenzyme 4, mitochondrial antibody
      • AK3 antibody
      • AK3L1 antibody
      • AK3L2 antibody
      • AK4 antibody
      • ATP AMP transphosphorylase antibody
      • ATP-AMP transphosphorylase antibody
      • GTP:AMP phosphotransferase antibody
      • KAD4_HUMAN antibody
      • MGC166959 antibody
      • mitochondrial adenylate kinase 3 antibody
      • nucleoside-triphosphate adenylate kinase antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling AK3L1 with ab131327 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-AK3L1 antibody [EPR7678] (ab131327) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunofluorescent analysis of HepG2 cells labelling AK3L1 with ab131327 at 1/250 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131327 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131327.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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