Recombinant
RabMAb

Recombinant Anti-AK3L1 antibody [EPR7679] (ab156880)

Overview

  • Product name

    Anti-AK3L1 antibody [EPR7679]
    See all AK3L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7679] to AK3L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human AK3L1 (P27144).

  • Positive control

    • Fetal liver extract, HepG2 cell lysate, Raji cell lysate, A431 cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    Images

    • Immunofluorescence of AK3L1 in A431 cells using ab156880 antibody at a dilution of 1/50.
    • All lanes : Anti-AK3L1 antibody [EPR7679] (ab156880) at 1/1000 dilution

      Lane 1 : Human fetal liver extract

      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 25 kDa

    References

    ab156880 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
    Specification
    total larvae, 4 days post fertilization
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. Evgenia Salta

    Verified customer

    Submitted Oct 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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