Anti-AK5 antibody [OTI1D6] (ab117927)

Submit a review Submit a question

Overview

  • Product name

    Anti-AK5 antibody [OTI1D6]
    See all AK5 primary antibodies

  • Description

    Mouse monoclonal [OTI1D6] to AK5

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human AK5 aa 1-562. NP_777283. Produced in HEK-293T cells.
    Sequence:

    MNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGG CDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPP IHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSL KIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTIT EIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRL KERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDA DRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDY EDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKY GFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVA SLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQR SRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDV FLQLCTAIDSIF


    Database link: Q9Y6K8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA. ICC/IF: COS-7 transiently transfected by pCMV6-ENTRY AK5 cDNA. FC: HEK-293T cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1.

  • General notes

    Clone OTI1D6 (formerly 1D6).

Properties

Applications

Our Abpromise guarantee covers the use of ab117927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP.

  • Tissue specificity

    Brain specific.

  • Sequence similarities

    Belongs to the adenylate kinase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adenylate kinase 5 antibody
      • Adenylate kinase 6 antibody
      • Adenylate kinase isoenzyme 5 antibody
      • AK 5 antibody
      • AK5 antibody
      • AK6 antibody
      • ATP AMP transphosphorylase 5 antibody
      • ATP-AMP transphosphorylase 5 antibody
      • KAD5 antibody
      • KAD5_HUMAN antibody
      • MGC33326 antibody
      see all

    Images

    • Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)
      Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)
      All lanes : Anti-AK5 antibody [OTI1D6] (ab117927) at 1/2000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
      Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 63 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)
      Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)

      pCMV6-ENTRY AK5 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for AK5 using ab117927 at a 1/100 dilution in ICC/IF.

    • Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)
      Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)

      Flow Cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1, using ab117927 (red) compared to an empty vector control plasmid (blue).

    References

    ab117927 has not yet been referenced specifically in any publications.

    Publishing research using ab117927? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab117927.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com