Key features and details
- Rabbit polyclonal to AKAP 95
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AKAP 95 antibody
See all AKAP 95 primary antibodies
DescriptionRabbit polyclonal to AKAP 95
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Gorilla
Synthetic peptide corresponding to Human AKAP 95 aa 642-692 (C terminal).
Database link: NP_005849.1
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAnchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
Tissue specificityHighly expressed in heart, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the AKAP95 family.
Contains 2 C2H2 AKAP95-type zinc fingers.
Cellular localizationNucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
- Information by UniProt
- A kinase (PRKA) anchor protein 8 antibody
- A kinase anchor protein 8 antibody
- A kinase anchor protein 95 antibody
All lanes : Anti-AKAP 95 antibody (ab72196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP 95 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
Lane 2 represents a Control IgG.
ab72196 has been referenced in 1 publication.
- Liu W et al. Roles of Cx43 and AKAP95 in ovarian cancer tissues in G1/S phase. Int J Clin Exp Pathol 8:14315-24 (2015). Human . PubMed: 26823747