Overview

  • Product name

  • Description

    Rabbit polyclonal to AKAP 95
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human AKAP 95 aa 642-692 (C terminal).
    Database link: NP_005849.1

  • Positive control

    • HeLa and 293T whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
  • Tissue specificity

    Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the AKAP95 family.
    Contains 2 C2H2 AKAP95-type zinc fingers.
  • Cellular localization

    Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • A kinase (PRKA) anchor protein 8 antibody
    • A kinase anchor protein 8 antibody
    • A kinase anchor protein 95 antibody
    • A kinase anchor protein 95 kDa antibody
    • A kinase anchor protein 95kDa antibody
    • A-kinase anchor protein 8 antibody
    • A-kinase anchor protein 95 kDa antibody
    • AKAP 8 antibody
    • AKAP 95 antibody
    • AKAP-8 antibody
    • AKAP-95 antibody
    • Akap8 antibody
    • AKAP8_HUMAN antibody
    • AKAP95 antibody
    • DKFZp586B1222 antibody
    see all

Images

  • All lanes : Anti-AKAP 95 antibody (ab72196) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 76 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP 95 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.

  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
    Lane 2 represents a Control IgG.

References

This product has been referenced in:

See 1 Publication for this product

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