Recombinant
RabMAb

Recombinant Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

Overview

  • Product name

    Anti-AKAP 95 antibody [EPR8978(B)]
    See all AKAP 95 primary antibodies
  • Description

    Rabbit monoclonal [EPR8978(B)] to AKAP 95
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AKAP 95 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, BxPC-3, Jurkat, HepG2, HeLa and Fetal liver lysates. Human tonsil and liver tissue. HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Dissociation constant (KD)

    KD = 1.29 x 10 -10 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8978(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
    • Tissue specificity

      Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the AKAP95 family.
      Contains 2 C2H2 AKAP95-type zinc fingers.
    • Cellular localization

      Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • A kinase (PRKA) anchor protein 8 antibody
      • A kinase anchor protein 8 antibody
      • A kinase anchor protein 95 antibody
      • A kinase anchor protein 95 kDa antibody
      • A kinase anchor protein 95kDa antibody
      • A-kinase anchor protein 8 antibody
      • A-kinase anchor protein 95 kDa antibody
      • AKAP 8 antibody
      • AKAP 95 antibody
      • AKAP-8 antibody
      • AKAP-95 antibody
      • Akap8 antibody
      • AKAP8_HUMAN antibody
      • AKAP95 antibody
      • DKFZp586B1222 antibody
      see all

    Images

    • All lanes : Anti-AKAP 95 antibody [EPR8978(B)] (ab134923) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : BxPC3 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : HepG2 lysate
      Lane 5 : HeLa lysate
      Lane 6 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 76 kDa

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue staining AKAP 95 with ab134923 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tonsil staining AKAP 95 with ab134923 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescence analysis of HepG2 cells staining AKAP 95 with ab134923 at a 1/100 dilution.

    • Immunohistochemical analysis using ab134923 showing positive staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab134923 showing positive staining in Normal heart tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab134923 showing positive staining in Normal colon tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134923 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134923.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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