Recombinant Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (ab249759)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free
See all AKAP associated Sperm Protein/ROPN1L primary antibodies -
Description
Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AKAP associated Sperm Protein/ROPN1L aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96C74 -
General notes
ab249759 is the carrier-free version of ab173298.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11266(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the ropporin family.
Contains 1 RIIa domain. - Information by UniProt
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Database links
- Entrez Gene: 83853 Human
- Omim: 611756 Human
- SwissProt: Q96C74 Human
- Unigene: 381089 Human
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Alternative names
- ropporin 1 like antibody
- AKAP-associated sperm protein antibody
- ASP antibody
see all
Images
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This data was developed using ab173298, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling AKAP associated Sperm Protein/ROPN1L with ab173298 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249759 has not yet been referenced specifically in any publications.