Recombinant Anti-AKAP1 antibody [EPR10518] (ab156004)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10518] to AKAP1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-AKAP1 antibody [EPR10518]
See all AKAP1 primary antibodies -
Description
Rabbit monoclonal [EPR10518] to AKAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human AKAP1 (Q92667).
-
Positive control
- SW480, Caco-2, and 293T cell lysates, 293T cells
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10518 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa).
|
Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa). |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. -
Tissue specificity
AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. -
Sequence similarities
Contains 1 KH domain.
Contains 1 Tudor domain. -
Domain
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8165 Human
- Omim: 602449 Human
- SwissProt: Q92667 Human
- Unigene: 463506 Human
-
Alternative names
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
see all
Images
-
All lanes : Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 149 kDa why is the actual band size different from the predicted? -
Flow Cytometrical analysis of permeabilized 293T cells with ab156004 antibody at a dilution of 1/100 (red) compared to a negative rabbit IgG (green).
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab156004 has not yet been referenced specifically in any publications.