Recombinant Anti-AKAP1 antibody [EPR10518] (ab156004)
![Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)](https://www.abcam.com/ps/products/156/ab156004/Images/AKAP1-Primary-antibodies-ab156004-1.jpg "Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10518] to AKAP1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AKAP1 antibody [EPR10518]
See all AKAP1 primary antibodies -
Description
Rabbit monoclonal [EPR10518] to AKAP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SW480, Caco-2, and 293T cell lysates, 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10518 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. -
Tissue specificity
AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. -
Sequence similarities
Contains 1 KH domain.
Contains 1 Tudor domain. -
Domain
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 8165 Human
- Omim: 602449 Human
- SwissProt: Q92667 Human
- Unigene: 463506 Human
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Alternative names
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
see all
Images
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Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)All lanes : Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 149 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-AKAP1 antibody [EPR10518] (ab156004)](https://www.abcam.com/ps/products/156/ab156004/Images/AKAP1-Primary-antibodies-ab156004-2.jpg)
Flow Cytometry (Intracellular) - Anti-AKAP1 antibody [EPR10518] (ab156004)Intracellular flow cytometrical analysis of permeabilized 293T cells with ab156004 antibody at a dilution of 1/100 (red) compared to a negative rabbit IgG (green).
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![Anti-AKAP1 antibody [EPR10518] (ab156004)](https://www.abcam.com/ps/products/156/ab156004/Images/ab156004-3-benefits-of-recombinant-antibodies.png)
Anti-AKAP1 antibody [EPR10518] (ab156004)
Datasheets and documents
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Datasheet download
References (1)
ab156004 has been referenced in 1 publication.
- Du Q et al. Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis. Oncol Lett 20:388 (2020). PubMed: 33193848
