RecombinantRabMAb

Recombinant Anti-AKAP1 antibody [EPR10518] (ab156004)

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Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)
  • Flow Cytometry - Anti-AKAP1 antibody [EPR10518] (ab156004)
  • Anti-AKAP1 antibody [EPR10518] (ab156004)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10518] to AKAP1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-AKAP1 antibody [EPR10518]
    See all AKAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10518] to AKAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human AKAP1 (Q92667).

  • Positive control

    • SW480, Caco-2, and 293T cell lysates, 293T cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10518

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.

    • Tissue specificity

      AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.

    • Sequence similarities

      Contains 1 KH domain.
      Contains 1 Tudor domain.

    • Domain

      RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

    • Cellular localization

      Mitochondrion outer membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • A kinase anchor protein antibody
        • A-kinase anchor protein 1 antibody
        • A-kinase anchor protein 149 kDa antibody
        • AKAP 149 antibody
        • AKAP antibody
        • Akap1 antibody
        • AKAP1_HUMAN antibody
        • D-AKAP-1 antibody
        • DAKAP1 antibody
        • Dual specificity A-kinase-anchoring protein 1 antibody
        • mitochondrial antibody
        • PRKA1 antibody
        • Protein kinase A-anchoring protein 1 antibody
        • Protein kinase A1 antibody
        • S-AKAP84 antibody
        • SAKAP84 antibody
        • Spermatid A-kinase anchor protein 84 antibody
        see all

      Images

      • Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)
        Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004)
        All lanes : Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution

        Lane 1 : SW480 cell lysate
        Lane 2 : Caco-2 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 97 kDa
        Observed band size: 149 kDa why is the actual band size different from the predicted?

      • Flow Cytometry - Anti-AKAP1 antibody [EPR10518] (ab156004)
        Flow Cytometry - Anti-AKAP1 antibody [EPR10518] (ab156004)
        Flow Cytometrical analysis of permeabilized 293T cells with ab156004 antibody at a dilution of 1/100 (red) compared to a negative rabbit IgG (green).
      • Anti-AKAP1 antibody [EPR10518] (ab156004)
        Anti-AKAP1 antibody [EPR10518] (ab156004)

      References (0)

      Publishing research using ab156004? Please let us know so that we can cite the reference in this datasheet.

      ab156004 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-AKAP1 antibody [EPR10518]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Dopaminergic Neurons, HCT116, HEK293T and HeLa cel)
      Gel Running Conditions
      Reduced Denaturing (NuPage 4-12% MES buffer)
      Loading amount
      30 µg
      Specification
      Dopaminergic Neurons, HCT116, HEK293T and HeLa cel
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 20 2020

      Western blot abreview for Anti-AKAP1 antibody [EPR10518]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (colon cancer)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      colon cancer
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 15 2020

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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