Product nameAnti-AKAP1 antibody [EPR10518]
See all AKAP1 primary antibodies
DescriptionRabbit monoclonal [EPR10518] to AKAP1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human AKAP1 (Q92667).
- SW480, Caco-2, and 293T cell lysates, 293T cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 97 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
Tissue specificityAKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
Sequence similaritiesContains 1 KH domain.
Contains 1 Tudor domain.
DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
All lanes : Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 149 kDa why is the actual band size different from the predicted?
Flow Cytometrical analysis of permeabilized 293T cells with ab156004 antibody at a dilution of 1/100 (red) compared to a negative rabbit IgG (green).
ab156004 has not yet been referenced specifically in any publications.