Anti-AKAP1 antibody - N-terminal (ab228875)
Key features and details
- Rabbit polyclonal to AKAP1 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKAP1 antibody - N-terminal
See all AKAP1 primary antibodies -
Description
Rabbit polyclonal to AKAP1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment within Human AKAP1 (N terminal). The exact sequence is proprietary.
Database link: Q92667 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 97 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/20000. Predicted molecular weight: 97 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. -
Tissue specificity
AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. -
Sequence similarities
Contains 1 KH domain.
Contains 1 Tudor domain. -
Domain
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 455150 Chimpanzee
- Entrez Gene: 8165 Human
- Omim: 602449 Human
- SwissProt: Q92667 Human
- Unigene: 463506 Human
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Alternative names
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
see all
Images
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Anti-AKAP1 antibody - N-terminal (ab228875) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for AKAP1 with ab228875 at 1/250 dilution in immunohistochemical analysis.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP1 (green) using ab228875 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228875 has not yet been referenced specifically in any publications.