Anti-AKAP1 antibody - N-terminal (ab228875)
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Overview
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Product name
Anti-AKAP1 antibody - N-terminal
See all AKAP1 primary antibodies -
Description
Rabbit polyclonal to AKAP1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human AKAP1 (N terminal). The exact sequence is proprietary.
Database link: Q92667 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/5000 - 1/20000. Predicted molecular weight: 97 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. -
Tissue specificity
AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. -
Sequence similarities
Contains 1 KH domain.
Contains 1 Tudor domain. -
Domain
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 455150 Chimpanzee
- Entrez Gene: 8165 Human
- Omim: 602449 Human
- SwissProt: Q92667 Human
- Unigene: 463506 Human
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Alternative names
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
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Images
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Western blot - Anti-AKAP1 antibody - N-terminal (ab228875)Anti-AKAP1 antibody - N-terminal (ab228875) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP1 antibody - N-terminal (ab228875)Paraffin-embedded human colon carcinoma tissue stained for AKAP1 with ab228875 at 1/250 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-AKAP1 antibody - N-terminal (ab228875)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP1 (green) using ab228875 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
References
ab228875 has not yet been referenced specifically in any publications.
