Key features and details
- Rabbit polyclonal to AKAP1 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AKAP1 antibody - N-terminal
See all AKAP1 primary antibodies
DescriptionRabbit polyclonal to AKAP1 - N-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human AKAP1 (N terminal). The exact sequence is proprietary.
Database link: Q92667
- WB: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 97 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
Tissue specificityAKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
Sequence similaritiesContains 1 KH domain.
Contains 1 Tudor domain.
DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- A kinase anchor protein antibody
- A-kinase anchor protein 1 antibody
- A-kinase anchor protein 149 kDa antibody
Anti-AKAP1 antibody - N-terminal (ab228875) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 97 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for AKAP1 with ab228875 at 1/250 dilution in immunohistochemical analysis.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP1 (green) using ab228875 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228875 has not yet been referenced specifically in any publications.