Overview

  • Product name

    Anti-AKAP1 antibody - N-terminal
    See all AKAP1 primary antibodies
  • Description

    Rabbit polyclonal to AKAP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human AKAP1 (N terminal). The exact sequence is proprietary.
    Database link: Q92667

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 97 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
  • Tissue specificity

    AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
  • Sequence similarities

    Contains 1 KH domain.
    Contains 1 Tudor domain.
  • Domain

    RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A kinase anchor protein antibody
    • A-kinase anchor protein 1 antibody
    • A-kinase anchor protein 149 kDa antibody
    • AKAP 149 antibody
    • AKAP antibody
    • Akap1 antibody
    • AKAP1_HUMAN antibody
    • D-AKAP-1 antibody
    • DAKAP1 antibody
    • Dual specificity A-kinase-anchoring protein 1 antibody
    • mitochondrial antibody
    • PRKA1 antibody
    • Protein kinase A-anchoring protein 1 antibody
    • Protein kinase A1 antibody
    • S-AKAP84 antibody
    • SAKAP84 antibody
    • Spermatid A-kinase anchor protein 84 antibody
    see all

Images

  • Anti-AKAP1 antibody - N-terminal (ab228875) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for AKAP1 with ab228875 at 1/250 dilution in immunohistochemical analysis.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP1 (green) using ab228875 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228875 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228875.
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