Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab9698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).Can be blocked with Human AKAP12 peptide (ab23159).|
FunctionAnchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Tissue specificityExpressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Sequence similaritiesContains 3 AKAP domains.
DomainPolybasic regions located between residues 266 and 557 are involved in binding PKC.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
- Information by UniProt
- A kinase (PRKA) anchor protein (gravin) 12 antibody
- A kinase Anchor Protein 12 antibody
- A kinase anchor protein 250kDa antibody
All lanes : Anti-AKAP12 antibody (ab9698) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 :
SW480 whole cell lysate (ab3957)
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 196 kDa
Observed band size: 268 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 268 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12.
This product has been referenced in:
- Lynch MJ et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005). Read more (PubMed: 16030021) »