Overview

  • Product name
    Anti-AKAP12 antibody [JP74]
    See all AKAP12 primary antibodies
  • Description
    Mouse monoclonal [JP74] to AKAP12
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).

  • Positive control
    • Rat brain cytosolic S1 fraction.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified Immunoglobulin
  • Clonality
    Monoclonal
  • Clone number
    JP74
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.02 - 0.04 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • Function
    Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • Tissue specificity
    Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • Sequence similarities
    Contains 3 AKAP domains.
  • Domain
    Polybasic regions located between residues 266 and 557 are involved in binding PKC.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein (gravin) 12 antibody
    • A kinase Anchor Protein 12 antibody
    • A kinase anchor protein 250kDa antibody
    • A-kinase anchor protein 12 antibody
    • A-kinase anchor protein 250 kDa antibody
    • AKA12_HUMAN antibody
    • AKAP 12 antibody
    • AKAP 250 antibody
    • AKAP-12 antibody
    • Akap12 antibody
    • AKAP250 antibody
    • DKFZp686M0430 antibody
    • DKFZp686O0331 antibody
    • FLJ20945 antibody
    • FLJ97621 antibody
    • Gercelin antibody
    • Gravin antibody
    • Kinase scaffold protein gravin antibody
    • Myasthenia gravis autoantigen antibody
    • Myasthenia gravis autoantigen gravin antibody
    • OTTHUMP00000017426 antibody
    • OTTHUMP00000017427 antibody
    • Src-Suppressed C Kinase Substrate antibody
    • Srcs5 antibody
    • SSeCKS antibody
    • Tsga12 antibody
    see all

References

This product has been referenced in:
  • Kanellopoulos AH  et al. Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene-targeted mice. EMBO J 37:427-445 (2018). Read more (PubMed: 29335280) »
  • Xue D  et al. Long noncoding RNA MALAT1 enhances the docetaxel resistance of prostate cancer cells via miR-145-5p-mediated regulation of AKAP12. J Cell Mol Med 22:3223-3237 (2018). Read more (PubMed: 29633510) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for your patience.

I sincerely apologize about this delay, but I have good news:

Yes, rat is a tested species and I have updated our datasheet to reflect that. We therefore guarantee this antibodyto work with rat samples. ...

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Thank you for contacting us.

I have received the following information from the lab so far:

Wedid a Blast comparison of the rat and human proteins and although the overall identity was only 68%, there are long stretches of >90%...

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Thank you for contacting us.

The lab is looking into this and will let me know if there is a reason why rat was not listed as tested species. I will get back to you when I have more information.

Thank you for your patience and unde...

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Thank you for contacting us and for alerting us to the problem you are experiencing with our Anti-AKAP12 antibody ab49849. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for contacting Abcam. I have looked at the western blot image that you attached and I believe that the Akap12 band is actually the large band a the top of the image, not marked by a red arrow. If you would like my opinion of which band is the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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