Overview

  • Product name
    Anti-AKAP12 antibody [JP74]
    See all AKAP12 primary antibodies
  • Description
    Mouse monoclonal [JP74] to AKAP12
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).

  • Positive control
    • Rat brain cytosolic S1 fraction.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified Immunoglobulin
  • Clonality
    Monoclonal
  • Clone number
    JP74
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.02 - 0.04 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • Function
    Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • Tissue specificity
    Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • Sequence similarities
    Contains 3 AKAP domains.
  • Domain
    Polybasic regions located between residues 266 and 557 are involved in binding PKC.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein (gravin) 12 antibody
    • A kinase Anchor Protein 12 antibody
    • A kinase anchor protein 250kDa antibody
    • A-kinase anchor protein 12 antibody
    • A-kinase anchor protein 250 kDa antibody
    • AKA12_HUMAN antibody
    • AKAP 12 antibody
    • AKAP 250 antibody
    • AKAP-12 antibody
    • Akap12 antibody
    • AKAP250 antibody
    • DKFZp686M0430 antibody
    • DKFZp686O0331 antibody
    • FLJ20945 antibody
    • FLJ97621 antibody
    • Gercelin antibody
    • Gravin antibody
    • Kinase scaffold protein gravin antibody
    • Myasthenia gravis autoantigen antibody
    • Myasthenia gravis autoantigen gravin antibody
    • OTTHUMP00000017426 antibody
    • OTTHUMP00000017427 antibody
    • Src-Suppressed C Kinase Substrate antibody
    • Srcs5 antibody
    • SSeCKS antibody
    • Tsga12 antibody
    see all

References

This product has been referenced in:
  • Kanellopoulos AH  et al. Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene-targeted mice. EMBO J 37:427-445 (2018). Read more (PubMed: 29335280) »
  • Xue D  et al. Long noncoding RNA MALAT1 enhances the docetaxel resistance of prostate cancer cells via miR-145-5p-mediated regulation of AKAP12. J Cell Mol Med 22:3223-3237 (2018). Read more (PubMed: 29633510) »
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your patience.

I sincerely apologize about this delay, but I have good news:

Yes, rat is a tested species and I have updated our datasheet to reflect that. We therefore guarantee this antibodyto work with rat samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

I have received the following information from the lab so far:

Wedid a Blast comparison of the rat and human proteins and although the overall identity was only 68%, there are long stretches of >90% identity throughout the two sequences, probably accounting for recognition of the rat protein by the antibody.

We are inquiring about why rat is not listed.

I will keep you informed once I receive more information.
Thank you for your patience.

Read More

Answer

Thank you for contacting us.

The lab is looking into this and will let me know if there is a reason why rat was not listed as tested species. I will get back to you when I have more information.

Thank you for your patience and understanding.

Read More

Answer

Thank you for contacting us and for alerting us to the problem you are experiencing with our Anti-AKAP12 antibody ab49849. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Indeed, this antibody has been tested in ICC/IF, and as we have received no complaints about this product, I am concerned it is not working for you. Thus, I would like to investigate this particular case further for you. In order to proceed with this, I have enclosed our questionnaire so that we can gather further information regarding the samples you tested and the protocol you used. I would appreciate if you could complete this, and also provide some information about the self-made antibody (e.g. which part of AKAP12 it recognises and which species it was raised in). I would also appreciate if you could also provide an image which would help us to assess the results.

Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. If we determine that the antibody is not working as stated on the datasheet, and as you have purchased it in December (i.e. in the last six months), we would be happy to replace or refund the antibody for you.

We look forward to receiving your reply.

Read More

Answer

Thank you for contacting Abcam. I have looked at the western blot image that you attached and I believe that the Akap12 band is actually the large band a the top of the image, not marked by a red arrow. If you would like my opinion of which band is the closet to 191kDa, then I would chose the top red arrow, but again I believe the actual Akap12 band is higher. Based on the datasheet and the references listed on the datasheet (PubMed: 21831305), the band that is of interest is at 300kDa. To achieve an image that has better resolution, you could rerun the gel and this time let the gel run longer, so that you get rid of all the low molecular weight products and gain a greater separation of the higher molecular weight proteins. Also using a molecular weight marker, that goes to 300kDa would also allow more accurate sizing. Please let me know if there is anything else I can help you with.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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