Question (36657) | Anti-AKAP12 antibody [JP74] (ab49849)

Go to datasheet (ab49849)

Question

Dear Sir or madam,
months ago we purchased AKAP12 antibody from Abcam (ab49849). In the antibody's datasheet is written that apart from WB and IP the antibody can be used for ICC (IF) as well. We have performed IF (fixation with paraformaldehyde (PFA) and permeabilization with Triton). This IF was compared with another IF where self-made antibody was used and it showed totally different staining! Since you haven’t shown any ICC pictures, neither you listed any references for IF I wonder whether the antibody was tested for ICC? I would appreciate any comment and/or recommendation.
Otherwise I have successful WB and IPs!
Looking forward for your reply

Answer

Thank you for contacting us and for alerting us to the problem you are experiencing with our Anti-AKAP12 antibody ab49849. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Indeed, this antibody has been tested in ICC/IF, and as we have received no complaints about this product, I am concerned it is not working for you. Thus, I would like to investigate this particular case further for you. In order to proceed with this, I have enclosed our questionnaire so that we can gather further information regarding the samples you tested and the protocol you used. I would appreciate if you could complete this, and also provide some information about the self-made antibody (e.g. which part of AKAP12 it recognises and which species it was raised in). I would also appreciate if you could also provide an image which would help us to assess the results.

Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. If we determine that the antibody is not working as stated on the datasheet, and as you have purchased it in December (i.e. in the last six months), we would be happy to replace or refund the antibody for you.

We look forward to receiving your reply.