Overview

  • Product name

  • Description

    Rabbit polyclonal to AKAP13/p47
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AKAP13/p47 (internal sequence).

  • Positive control

    • IHC-P: Human lung carcinoma tissue. WB: Extracts from Jurkat and A549 cells. ICC/IF: formaldehyde fixed A549 cells.
  • General notes

     This product was previously labelled as AKAP13

     

Properties

Applications

Our Abpromise guarantee covers the use of ab74048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 308 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
  • Tissue specificity

    Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • Domain

    Both the DH and PH domains are required for transforming activity.
  • Cellular localization

    Cytoplasm; Cytoplasm. Nucleus and Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A kinase (PRKA) anchor protein 13 antibody
    • A kinase anchor protein 13 antibody
    • A kinase anchoring protein antibody
    • A-kinase anchor protein 13 antibody
    • AKAP 13 antibody
    • AKAP Lbc antibody
    • AKAP-13 antibody
    • AKAP-Lbc antibody
    • AKAP13 antibody
    • AKP13_HUMAN antibody
    • ARHGEF13 antibody
    • Breast cancer nuclear receptor binding auxiliary protein antibody
    • Breast cancer nuclear receptor-binding auxiliary protein antibody
    • BRX antibody
    • c lbc antibody
    • FLJ11952 antibody
    • FLJ43341 antibody
    • Guanine nucleotide exchange factor Lbc antibody
    • HA 3 antibody
    • HA3 antibody
    • Ht 31 antibody
    • Ht31 antibody
    • Human thyroid anchoring protein 31 antibody
    • Human thyroid-anchoring protein 31 antibody
    • LBC antibody
    • LBC oncogene antibody
    • Lymphoid blast crisis oncogene antibody
    • Non oncogenic Rho GTPase specific GTP exchange factor antibody
    • Non-oncogenic Rho GTPase-specific GTP exchange factor antibody
    • p47 antibody
    • Protein kinase A anchoring protein 13 antibody
    • Protein kinase A-anchoring protein 13 antibody
    • PROTO LB antibody
    • PROTO LBC antibody
    see all

Images

  • All lanes : Anti-AKAP13/p47 antibody (ab74048) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from A549 cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 308 kDa

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74048, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • ICC/IF image of ab74048 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74048 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab74048 has not yet been referenced specifically in any publications.

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