Key features and details
- Rabbit polyclonal to AKAP13/p47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AKAP13/p47 antibody
See all AKAP13/p47 primary antibodies
DescriptionRabbit polyclonal to AKAP13/p47
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human AKAP13/p47 aa 2754-2804.
Database link: NP_009131.2
- HeLa whole cell lysate (ab150035)
General notesImmunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
This product was previously labelled as AKAP13
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 308 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionAnchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
Tissue specificityIsoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
DomainBoth the DH and PH domains are required for transforming activity.
Cellular localizationCytoplasm; Cytoplasm. Nucleus and Membrane.
- Information by UniProt
- A kinase (PRKA) anchor protein 13 antibody
- A kinase anchor protein 13 antibody
- A kinase anchoring protein antibody
All lanes : Anti-AKAP13/p47 antibody (ab99377) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 308 kDa
Exposure time: 10 seconds
Lane 1 : Detection of Human AKAP13/p47 by Immunoprecipitation from HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded) using 3µg/mg lysate ab99377 lane 2 : Control IgG
ab99377 has been referenced in 1 publication.
- Schrade K et al. An AKAP-Lbc-RhoA interaction inhibitor promotes the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells. PLoS One 13:e0191423 (2018). PubMed: 29373579