Overview

  • Product name

    Anti-AKAP4 antibody [EPR21123]
    See all AKAP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR21123] to AKAP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human AKAP4 aa 1-150. The exact sequence is proprietary.
    Database link: Q5JQC9

  • Positive control

    • WB: Human testis lysate. IHC-P: Human testis tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21123
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 94 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
  • Tissue specificity

    Testis specific; only expressed in round spermatids.
  • Sequence similarities

    Belongs to the AKAP110 family.
  • Developmental stage

    Post-meiotic phase of spermatogenesis.
  • Domain

    RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization

    Cell projection > cilium > flagellum. Localizes to the principle piece of the sperm flagellum.
  • Information by UniProt
  • Database links

  • Alternative names

    • A kinase (PRKA) anchor protein 4 antibody
    • A kinase anchor protein 4 antibody
    • A kinase anchor protein 82 kDa antibody
    • A-kinase anchor protein 4 antibody
    • A-kinase anchor protein 82 kDa antibody
    • AKAP 4 antibody
    • AKAP 82 antibody
    • AKAP-4 antibody
    • AKAP4 antibody
    • AKAP4_HUMAN antibody
    • AKAP82 antibody
    • FSC 1 antibody
    • FSC1 antibody
    • hAKAP 82 antibody
    • hAKAP82 antibody
    • HI antibody
    • Major sperm fibrous sheath protein antibody
    • p82 antibody
    • PRKA 4 antibody
    • PRKA4 antibody
    • Protein kinase A anchoring protein 4 antibody
    • Protein kinase A-anchoring protein 4 antibody
    • Testis specific gene HI antibody
    see all

Images

  • Anti-AKAP4 antibody [EPR21123] (ab230954) at 1/1000 dilution + Human testis lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa


    Exposure time: 6 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling AKAP4 with ab230954 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in spermatids of human testis (PMID:19139142) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling AKAP4 with ab230954 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) Secondary antibody.

    Negative control: Negative staining in human lung (PMID: 19139142).

    Counterstained with hematoxylin.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab230954 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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