Key features and details
- Mouse monoclonal [OTI6F7] to AKAP7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-AKAP7 antibody [OTI6F7]
See all AKAP7 primary antibodies
DescriptionMouse monoclonal [OTI6F7] to AKAP7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human AKAP7 aa 1-348. Produced in E. coli. NP_057461
MERPEAGGINSNECENVSRKKKMSEEFEANTMDSLVDMPFATVDIQDDCG ITDEPQINLKRSQENEWVKSDQVKKRKKKRKDYQPNYFLSIPITNKEIIK GIKILQNAIIQQDERLAKAMVSDGSFHITLLVMQLLNEDEVNIGIDALLE LKPFIEELLQGKHLTLPFQGIGTFGNQVGFVKLAEGDHVNSLLEIAETAN RTFQEKGILVGESRSFKPHLTFMKLSKSPWLRKNGVKKIDPDLYEKFISH RFGEEILYRIDLCSMLKKKQSNGYYHCESSIVIGEKNGGEPDDAELVRLS KRLVENAVLKAVQQYLEETQNKNKPGEGSSVKTEAADQNGNDNENNRK
Database link: Q9P0M2
- IHC-P: Human tonsil tissue. WB: pCMV6-ENTRY AKAP7 transfected HEK-293T cell lysate.
Clone OTI6F7 (formerly 6F7).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 11 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTargets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel.
Tissue specificityExpressed in brain, heart, lung, pancreas and skeletal muscle.
Cellular localizationApical cell membrane. Targeted predominantly to the apical membrane and Lateral cell membrane. Targeted predominantly to the lateral membrane.
- Information by UniProt
- A kinase (PRKA) anchor protein 7 antibody
- A kinase anchor protein 18 kD antibody
- A kinase anchor protein 7 antibody
Paraffin-embedded human tonsil tissue stained for AKAP7 with ab236372 at 1/150 dilution in immunohistochemical analysis.
All lanes : Anti-AKAP7 antibody [OTI6F7] (ab236372) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY AKAP7 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 11 kDa
Cells transfected for 48 hours then lysed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236372 has not yet been referenced specifically in any publications.