Anti-AKAP9 antibody [17G10] (ab32679)
Key features and details
- Mouse monoclonal [17G10] to AKAP9
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKAP9 antibody [17G10]
See all AKAP9 primary antibodies -
Description
Mouse monoclonal [17G10] to AKAP9 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Chimpanzee -
Immunogen
Synthetic peptide:
QFRQRKAQSDGQSPS
, corresponding to amino acids 31-45 of Human AKAP9 -
Positive control
- ICC: A431, HeLa and NIH-3T3 cells. IHC-P: Human pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
17G10 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/10 - 1/100.
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ICC |
Use a concentration of 4 µg/ml.
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Notes |
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IHC-P
1/10 - 1/100. |
ICC
Use a concentration of 4 µg/ml. |
Target
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Function
Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. -
Tissue specificity
Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas. -
Involvement in disease
Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. -
Domain
RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells. - Information by UniProt
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Database links
- Entrez Gene: 10142 Human
- Entrez Gene: 100986 Mouse
- Omim: 604001 Human
- SwissProt: Q99996 Human
- SwissProt: Q70FJ1 Mouse
- Unigene: 651221 Human
- Unigene: 46044 Mouse
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Alternative names
- A kinase (PRKA) anchor protein (yotiao) 9 antibody
- A kinase (PRKA) anchor protein 9 antibody
- A kinase anchor protein 350kDa antibody
see all
Images
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Immunocytochemical analysis of AKAP9 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemical analysis of AKAP9 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemical analysis of AKAP9 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab32679 has been referenced in 1 publication.
- Wu S et al. AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway. Cancer Manag Res 14:157-167 (2022). PubMed: 35046723