Overview

  • Product name

  • Description

    Rabbit polyclonal to AKAP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AKAP9 aa 71-191.
    Database link: Q99996

  • Positive control

    • IHC-P: Human lung cancer and colon cancer tissues. ICC/IF: HeLa cells. WB: Recombinant AKAP9 protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 454 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
  • Tissue specificity

    Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
  • Involvement in disease

    Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
  • Domain

    RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • A kinase (PRKA) anchor protein (yotiao) 9 antibody
    • A kinase (PRKA) anchor protein 9 antibody
    • A kinase anchor protein 350kDa antibody
    • A kinase anchor protein 450 kDa antibody
    • A kinase anchor protein 9 antibody
    • A kinase anchoring protein 350 antibody
    • A kinase anchoring protein 450 antibody
    • A-kinase anchor protein 350 kDa antibody
    • A-kinase anchor protein 450 kDa antibody
    • A-kinase anchor protein 9 antibody
    • AKAP 120-like protein antibody
    • AKAP 350 antibody
    • AKAP 450 antibody
    • AKAP 9 antibody
    • AKAP-9 antibody
    • AKAP120 like protein antibody
    • AKAP350 antibody
    • AKAP450 antibody
    • AKAP9 antibody
    • AKAP9_HUMAN antibody
    • Centrosome and Golgi localized PKN associated protein antibody
    • Centrosome and golgi localized protein antibody
    • Centrosome- and Golgi-localized PKN-associated protein antibody
    • CG NAP antibody
    • CG-NAP antibody
    • hgAKAP 350 antibody
    • hgAKAP350 antibody
    • Hyperion antibody
    • KIAA0803 antibody
    • Kinase N associated protein antibody
    • MU RMS 40.16A antibody
    • PRKA 9 antibody
    • PRKA9 antibody
    • Protein hyperion antibody
    • Protein kinase A anchoring protein 9 antibody
    • Protein kinase A-anchoring protein 9 antibody
    • Protein yotiao antibody
    • Yotiao antibody
    see all

Images

  • All lanes : Anti-AKAP9 antibody (ab237752) at 1/500 dilution

    Lane 1 : Recombinant AKAP9 protein at 0.08 µg
    Lane 2 : Recombinant AKAP9 protein at 0.04 µg
    Lane 3 : Recombinant AKAP9 protein at 0.02 µg
    Lane 4 : Recombinant AKAP9 protein at 0.01 µg

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 454 kDa

  • Paraffin-embedded human lung cancer tissue stained for AKAP9 using ab237752 at 1/100 dilution in immunohistochemical analysis.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP9 (green) using ab237752 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human colon cancer tissue stained for AKAP9 using ab237752 at 1/100 dilution in immunohistochemical analysis.

References

ab237752 has not yet been referenced specifically in any publications.

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