Key features and details
- Rabbit polyclonal to AKAP9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AKAP9 antibody
See all AKAP9 primary antibodies
DescriptionRabbit polyclonal to AKAP9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human AKAP9 aa 71-191.
Database link: Q99996
- IHC-P: Human lung cancer and colon cancer tissues. ICC/IF: HeLa cells. WB: Recombinant AKAP9 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 454 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionBinds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
Tissue specificityWidely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
Involvement in diseaseDefects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
DomainRII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells.
- Information by UniProt
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All lanes : Anti-AKAP9 antibody (ab237752) at 1/500 dilution
Lane 1 : Recombinant AKAP9 protein at 0.08 µg
Lane 2 : Recombinant AKAP9 protein at 0.04 µg
Lane 3 : Recombinant AKAP9 protein at 0.02 µg
Lane 4 : Recombinant AKAP9 protein at 0.01 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 454 kDa
Paraffin-embedded human lung cancer tissue stained for AKAP9 using ab237752 at 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP9 (green) using ab237752 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human colon cancer tissue stained for AKAP9 using ab237752 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237752 has not yet been referenced specifically in any publications.