Product nameAnti-AKR1A1 antibody
See all AKR1A1 primary antibodies
DescriptionGoat polyclonal to AKR1A1
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- Recombinant Human AKR1A1 protein (ab95472) can be used as a positive control in WB. Human Placenta and Liver lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab11802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||1/16000. This application has only been tested against the recombinant protein.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human AKR1A1 peptide (ab23210). Approx 37-40kDa band observed in Human Placenta and Liver lysates.|
FunctionCatalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).
Tissue specificityWidely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- AK1A1_HUMAN antibody
- Akr1a1 antibody
- Alcohol dehydrogenase [NADP+] antibody
ab11802 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Anti-AKR1A1 antibody (ab11802) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab11802 at 2.5µg/ml, staining AKR1A1 in Human Kidney sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and AP-staining was used.
This product has been referenced in:
- Singh S et al. SMAR1 regulates free radical stress through modulation of AKR1a4 enzyme activity. Int J Biochem Cell Biol 42:1105-14 (2010). Read more (PubMed: 20097305) »
- Diggle CP et al. Ketohexokinase: expression and localization of the principal fructose-metabolizing enzyme. J Histochem Cytochem 57:763-74 (2009). Read more (PubMed: 19365088) »