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    akr1b10-antibody-ab61832.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-AKR1B10 antibody (ab61832)

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Western blot - Anti-AKR1B10 antibody (ab61832)

    Key features and details

    • Rabbit polyclonal to AKR1B10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human AKR1B10 protein (ab85415)

    View more associated products

    Overview

    • Product name

      Anti-AKR1B10 antibody
      See all AKR1B10 primary antibodies
    • Description

      Rabbit polyclonal to AKR1B10
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human AKR1B10 aa 50-150 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab97989)

    • Positive control

      • Recombinant Human AKR1B10 protein (ab85415) can be used as a positive control in WB. This antibody gave a positive signal in A549 whole cell lysate and Human small intestine tissue lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • Other
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Human AKR1B10 protein (ab85415)
    • Recombinant Protein

      • Recombinant Human AKR1B10 protein (ab85415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab61832 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

    Target

    • Function

      Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
    • Tissue specificity

      Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
    • Sequence similarities

      Belongs to the aldo/keto reductase family.
    • Cellular localization

      Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
    • Target information above from: UniProt accession O60218 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57016 Human
      • Omim: 604707 Human
      • SwissProt: O60218 Human
      • Unigene: 116724 Human
      • Alternative names

        • AK1BA_HUMAN antibody
        • AKR1B10 antibody
        • AKR1B11 antibody
        • AKR1B12 antibody
        • Aldo keto reductase family 1 member B10 antibody
        • aldo keto reductase family 1 member B11 antibody
        • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
        • Aldo-keto reductase family 1 member B10 antibody
        • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
        • aldose reductase like 1 antibody
        • Aldose reductase like antibody
        • aldose reductase like peptide antibody
        • Aldose reductase related protein antibody
        • Aldose reductase-like antibody
        • Aldose reductase-related protein antibody
        • ALDRLn antibody
        • ARL 1 antibody
        • ARL-1 antibody
        • ARL1 antibody
        • ARL1 Human small intestine reductase antibody
        • ARP antibody
        • hARP antibody
        • HIS antibody
        • HSI antibody
        • SI reductase antibody
        • Small intestine reductase antibody
        see all

      Images

      • Western blot - Anti-AKR1B10 antibody (ab61832)
        Western blot - Anti-AKR1B10 antibody (ab61832)
        All lanes : Anti-AKR1B10 antibody (ab61832) at 1 µg/ml

        Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
        Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 36 kDa
        Observed band size: 36 kDa
        Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 10 minutes

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab61832? Please let us know so that we can cite the reference in this datasheet.

      ab61832 has not yet been referenced specifically in any publications.

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