Anti-AKR1B10 antibody (ab61832)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AKR1B10 antibody
See all AKR1B10 primary antibodies -
Description
Rabbit polyclonal to AKR1B10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AKR1B10 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97989) -
Positive control
- Recombinant Human AKR1B10 protein (ab85415) can be used as a positive control in WB. This antibody gave a positive signal in A549 whole cell lysate and Human small intestine tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab61832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
Tissue specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
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Database links
- Entrez Gene: 57016 Human
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
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Alternative names
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
see all
Images
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Western blot - Anti-AKR1B10 antibody (ab61832)All lanes : Anti-AKR1B10 antibody (ab61832) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Protocols
Datasheets and documents
References
ab61832 has not yet been referenced specifically in any publications.
