Anti-AKR1B10 antibody (ab96417)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-AKR1B10 antibody
See all AKR1B10 primary antibodies -
Description
Rabbit polyclonal to AKR1B10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-286 of Human AKR1B10 (NP_064695).
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Positive control
- MOLT4 and Raji whole cell lysates; HeLa cells; OVCAR3 xenograft; 293T, A431, H1299 and HepG2 cell lines
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
Tissue specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
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Database links
- Entrez Gene: 57016 Human
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
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Alternative names
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
see all
Images
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Western blot - Anti-AKR1B10 antibody (ab96417)All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody (ab96417)ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)ab96417, at 1/500 dilution, staining AKR1B10 in paraffin-embedded OVCAR3 xenograft sections by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)This image is courtesy of an anonymous Abreviewab96417 staining AKR1B10 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Goat anti-rabbit IgG + IgM H+L polyclonal (1/100) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Ghosh A et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . Read more (PubMed: 29481290) »
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . Read more (PubMed: 29018201) »
