Anti-AKR1B10 antibody (ab96417)
Key features and details
- Rabbit polyclonal to AKR1B10
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-AKR1B10 antibody
See all AKR1B10 primary antibodies -
Description
Rabbit polyclonal to AKR1B10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human AKR1B10 aa 1-286.
Database link: O60218 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/10000. Predicted molecular weight: 36 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
Tissue specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
-
Database links
- Entrez Gene: 57016 Human
- Entrez Gene: 67861 Mouse
- Entrez Gene: 296972 Rat
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
- Unigene: 70371 Mouse
- Unigene: 228771 Rat
-
Alternative names
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
see all
Images
-
Western blot - Anti-AKR1B10 antibody (ab96417)Anti-AKR1B10 antibody (ab96417) at 1/5000 dilution + Mouse colon lysate at 50 µg
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)All lanes : Anti-AKR1B10 antibody (ab96417) at 1/2500 dilution
Lane 1 : Jurkat cells
Lane 2 : Raji cells
Lane 3 : NCI-H929 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)Anti-AKR1B10 antibody (ab96417) at 1/50000 dilution + Rat colon lysate at 50 µg
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody (ab96417)ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
Protocols
References (3)
ab96417 has been referenced in 3 publications.
- Ghosh A et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . PubMed: 29481290
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201
- Hevir N et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . PubMed: 23146748
