Overview

  • Product name
    Anti-AKR1B10 antibody
    See all AKR1B10 primary antibodies
  • Description
    Rabbit polyclonal to AKR1B10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-286 of Human AKR1B10 (NP_064695).

  • Positive control
    • MOLT4 and Raji whole cell lysates; HeLa cells; OVCAR3 xenograft; 293T, A431, H1299 and HepG2 cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab96417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Tissue specificity
    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt
  • Database links
    • Alternative names
      • AK1BA_HUMAN antibody
      • AKR1B10 antibody
      • AKR1B11 antibody
      • AKR1B12 antibody
      • Aldo keto reductase family 1 member B10 antibody
      • aldo keto reductase family 1 member B11 antibody
      • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
      • Aldo-keto reductase family 1 member B10 antibody
      • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
      • aldose reductase like 1 antibody
      • Aldose reductase like antibody
      • aldose reductase like peptide antibody
      • Aldose reductase related protein antibody
      • Aldose reductase-like antibody
      • Aldose reductase-related protein antibody
      • ALDRLn antibody
      • ARL 1 antibody
      • ARL-1 antibody
      • ARL1 antibody
      • ARL1 Human small intestine reductase antibody
      • ARP antibody
      • hARP antibody
      • HIS antibody
      • HSI antibody
      • SI reductase antibody
      • Small intestine reductase antibody
      see all

    Images

    • Western blot - Anti-AKR1B10 antibody (ab96417)
      Western blot - Anti-AKR1B10 antibody (ab96417)
      All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution

      Lane 1 : MOLT4 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 36 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody (ab96417)
      Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody (ab96417)
      ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)
      ab96417, at 1/500 dilution, staining AKR1B10 in paraffin-embedded OVCAR3 xenograft sections by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody (ab96417)This image is courtesy of an anonymous Abreview
      ab96417 staining AKR1B10 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Goat anti-rabbit IgG + IgM H+L polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Huppke P  et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . Read more (PubMed: 29018201) »
    • Hevir N  et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . Read more (PubMed: 23146748) »

    See all 2 Publications for this product

    Publishing research using ab96417? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AKR1B10 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (endometrial cancer)
    Specification
    endometrial cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 13 2013

    Western blot abreview for Anti-AKR1B10 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
    Loading amount
    30 µg
    Specification
    endometrial cancer and adjacent control endometriu
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 12 2013

    Dear all,

    I want to purchase AKR1B1 and AKR1B10 antibodies. I have allready selected them on your webside, but then I blasted both proteins and I found thet they have 70.6% identity and 91.1% similarity. Do you think they are similar enough ...

    Read More

    Thank you for your inquiry.
    I was in contact with the lab and unfortunately, we did not experimentally test if there is a cross reaction of the antibody to the other proteins.

    In general, we do not expect any cross reactivity if the homo...

    Read More

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