Recombinant
RabMAb

Recombinant Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

Overview

  • Product name

    Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free
    See all AKR1B10 primary antibodies
  • Description

    Rabbit monoclonal [EPR14421] to AKR1B10 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AKR1B10 aa 100-200. The exact sequence is proprietary.
    Database link: O60218

  • Positive control

    • IHC-P: Human stomach tissue.
  • General notes

    Ab232623 is the carrier-free version of ab192865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232623 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Tissue specificity

    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • AK1BA_HUMAN antibody
    • AKR1B10 antibody
    • AKR1B11 antibody
    • AKR1B12 antibody
    • Aldo keto reductase family 1 member B10 antibody
    • aldo keto reductase family 1 member B11 antibody
    • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
    • Aldo-keto reductase family 1 member B10 antibody
    • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
    • aldose reductase like 1 antibody
    • Aldose reductase like antibody
    • aldose reductase like peptide antibody
    • Aldose reductase related protein antibody
    • Aldose reductase-like antibody
    • Aldose reductase-related protein antibody
    • ALDRLn antibody
    • ARL 1 antibody
    • ARL-1 antibody
    • ARL1 antibody
    • ARL1 Human small intestine reductase antibody
    • ARP antibody
    • hARP antibody
    • HIS antibody
    • HSI antibody
    • SI reductase antibody
    • Small intestine reductase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling AKR1B10 with ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cells labeling AKR1B10 with ab192865 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

  • Flow cytometric analysis of A549 cells labeling AKR1B10 using ab192865 at a 1/50 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

  • Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling AKR1B10 using ab192865 at a 1/1,000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab232623 has not yet been referenced specifically in any publications.

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