Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9767(B)] to AKR1B10
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Human
Product nameAnti-AKR1B10 antibody [EPR9767(B)]
See all AKR1B10 primary antibodies
DescriptionRabbit monoclonal [EPR9767(B)] to AKR1B10
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human AKR1B10 aa 300-400 (C terminal). The exact sequence is proprietary.
- A549 cells; Human small intestine and A549 cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab139685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
FunctionActs as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Tissue specificityFound in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Sequence similaritiesBelongs to the aldo/keto reductase family.
Cellular localizationLysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
- Information by UniProt
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
All lanes : Anti-AKR1B10 antibody [EPR9767(B)] (ab139685) at 1/1000 dilution
Lane 1 : Human small intestine cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Immunofluorescent analysis of A549 cells labeling AKR1B10 with ab139685 at 1/100 dilution.
ab139685 has been referenced in 1 publication.
- Cong Z et al. Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98. Cell Death Dis 10:84 (2019). PubMed: 30692511