Anti-AKR1C1 antibody (ab192785)
Key features and details
- Rabbit polyclonal to AKR1C1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-AKR1C1 antibody
See all AKR1C1 primary antibodies -
Description
Rabbit polyclonal to AKR1C1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human AKR1C1 aa 22-248. The exact sequence is proprietary.
Database link: Q04828 -
Positive control
- Human liver tissue; A431 cells; Raji whole cell lysate; mouse liver tissue lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: 20% Glycerol, PBS, 0.00038% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00038% 2-methyl-4-isothiazolin-3-one -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab192785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 37 kDa. | |
| IHC-P | Use a concentration of 7.5 µg/ml. |
Target
-
Function
Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. -
Tissue specificity
Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 1645 Human
- Omim: 600449 Human
- SwissProt: Q04828 Human
- Unigene: 460260 Human
-
Alternative names
- 2-dihydrobenzene-1 antibody
- 2-diol dehydrogenase antibody
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
see all
Images
-
Western blot - Anti-AKR1C1 antibody (ab192785)Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 37 kDa10% SDS PAGE
-
Western blot - Anti-AKR1C1 antibody (ab192785)Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + mouse liver tissue lysate at 50 µg
Predicted band size: 37 kDa10% SDS-PAGE
-
Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody (ab192785)Immunofluorescence analysis of A431 cells, labeling AKR1C1 (green, upper panel) with ab192785 at 1/200 dilution. Nuclei were counterstained with Hoechst 33342 (blue, lower panel).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C1 antibody (ab192785)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue, labeling AKR1C1 with ab192785 at 7.5 μg/ml.
Protocols
References (2)
ab192785 has been referenced in 2 publications.
- Narzt MS et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol 20:467-482 (2019). PubMed: 30466060
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201
