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    akr1c1-antibody-ab192785.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-AKR1C1 antibody (ab192785)

  • Datasheet
  • SDS
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Western blot - Anti-AKR1C1 antibody (ab192785)
  • Western blot - Anti-AKR1C1 antibody (ab192785)
  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody (ab192785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C1 antibody (ab192785)

Key features and details

  • Rabbit polyclonal to AKR1C1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-AKR1C1 antibody
    See all AKR1C1 primary antibodies
  • Description

    Rabbit polyclonal to AKR1C1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human AKR1C1 aa 22-248. The exact sequence is proprietary.
    Database link: Q04828

  • Positive control

    • Human liver tissue; A431 cells; Raji whole cell lysate; mouse liver tissue lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Constituents: 20% Glycerol, PBS, 0.00038% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00038% 2-methyl-4-isothiazolin-3-one
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Developmental Biology
    • Reproduction
    • Hormones
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • Raji whole cell lysate (ab30124)
  • Related Products

    • Recombinant Human AKR1C1 protein (ab93730)

Applications

Our Abpromise guarantee covers the use of ab192785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 7.5 µg/ml.

Target

  • Function

    Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
  • Tissue specificity

    Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q04828 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1645 Human
    • Omim: 600449 Human
    • SwissProt: Q04828 Human
    • Unigene: 460260 Human
    • Alternative names

      • 2-dihydrobenzene-1 antibody
      • 2-diol dehydrogenase antibody
      • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
      • 20 ALPHA HSD antibody
      • 20 alpha hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • 20-alpha-hydroxysteroid dehydrogenase antibody
      • 20ALPHAHSD antibody
      • 2ALPHAHSD antibody
      • AK1C1 antibody
      • AK1C1_HUMAN antibody
      • AKR1C1 antibody
      • Aldo keto reductase family 1 member C1 antibody
      • aldo-keto reductase C antibody
      • Aldo-keto reductase family 1 member C1 antibody
      • Aldo-keto reductase family, 1 member 1 antibody
      • C9 antibody
      • Chlordecone reductase homolog antibody
      • Chlordecone reductase homolog HAKRC antibody
      • DD1 antibody
      • DD1/DD2 antibody
      • DDH antibody
      • DDH1 antibody
      • Dihydrodiol dehydrogenase 1 antibody
      • Dihydrodiol dehydrogenase 1/2 antibody
      • dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase antibody
      • dihydrodiol dehydrogenase isoform DD1 antibody
      • Dihydrodiol dehydrogenase, type 1 antibody
      • H37 antibody
      • HAKRC antibody
      • HBAB antibody
      • Hepatic dihydrodiol dehydrogenase antibody
      • High affinity hepatic bile acid-binding protein antibody
      • High-affinity hepatic bile acid-binding protein antibody
      • Indanol dehydrogenase antibody
      • MBAB antibody
      • MGC8954 antibody
      • Trans-1 antibody
      • Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibody
      • Type II 3 alpha hydroxysteroid dehydrogenase antibody
      see all

    Images

    • Western blot - Anti-AKR1C1 antibody (ab192785)
      Western blot - Anti-AKR1C1 antibody (ab192785)
      Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + Raji whole cell lysate at 30 µg

      Predicted band size: 37 kDa



      10% SDS PAGE

    • Western blot - Anti-AKR1C1 antibody (ab192785)
      Western blot - Anti-AKR1C1 antibody (ab192785)
      Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + mouse liver tissue lysate at 50 µg

      Predicted band size: 37 kDa



      10% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody (ab192785)
      Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody (ab192785)

      Immunofluorescence analysis of A431 cells, labeling AKR1C1 (green, upper panel) with ab192785 at 1/200 dilution. Nuclei were counterstained with Hoechst 33342 (blue, lower panel).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C1 antibody (ab192785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C1 antibody (ab192785)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue, labeling AKR1C1 with ab192785 at 7.5 μg/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab192785? Please let us know so that we can cite the reference in this datasheet.

    ab192785 has been referenced in 2 publications.

    • Narzt MS  et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol 20:467-482 (2019). PubMed: 30466060
    • Huppke P  et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab192785.
    Please use the links above to contact us or submit feedback about this product.

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