Key features and details
- Rabbit polyclonal to AKR1C1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AKR1C1 antibody
See all AKR1C1 primary antibodies
DescriptionRabbit polyclonal to AKR1C1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human AKR1C1 aa 22-248. The exact sequence is proprietary.
Database link: Q04828
- Human liver tissue; A431 cells; Raji whole cell lysate; mouse liver tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Constituents: 20% Glycerol, PBS, 0.00038% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00038% 2-methyl-4-isothiazolin-3-one
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 7.5 µg/ml.|
FunctionConverts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
Tissue specificityExpressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- 2-dihydrobenzene-1 antibody
- 2-diol dehydrogenase antibody
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS PAGE
Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + mouse liver tissue lysate at 50 µg
Predicted band size: 37 kDa
Immunofluorescence analysis of A431 cells, labeling AKR1C1 (green, upper panel) with ab192785 at 1/200 dilution. Nuclei were counterstained with Hoechst 33342 (blue, lower panel).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue, labeling AKR1C1 with ab192785 at 7.5 μg/ml.
ab192785 has been referenced in 2 publications.
- Narzt MS et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol 20:467-482 (2019). PubMed: 30466060
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201