Anti-AKR1C1 antibody (ab192785)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-AKR1C1 antibody
See all AKR1C1 primary antibodies -
Description
Rabbit polyclonal to AKR1C1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human AKR1C1 aa 22-248. The exact sequence is proprietary.
Database link: Q04828 -
Positive control
- Human liver tissue; A431 cells; Raji whole cell lysate; mouse liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: 20% Glycerol, PBS, 0.00038% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00038% 2-methyl-4-isothiazolin-3-one -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab192785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 37 kDa. | |
| IHC-P | Use a concentration of 7.5 µg/ml. |
Target
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Function
Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. -
Tissue specificity
Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1645 Human
- Omim: 600449 Human
- SwissProt: Q04828 Human
- Unigene: 460260 Human
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Alternative names
- 2-dihydrobenzene-1 antibody
- 2-diol dehydrogenase antibody
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
see all
Images
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Western blot - Anti-AKR1C1 antibody (ab192785)Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 37 kDa10% SDS PAGE
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Western blot - Anti-AKR1C1 antibody (ab192785)Anti-AKR1C1 antibody (ab192785) at 1/1000 dilution + mouse liver tissue lysate at 50 µg
Predicted band size: 37 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1 antibody (ab192785)Immunofluorescence analysis of A431 cells, labeling AKR1C1 (green, upper panel) with ab192785 at 1/200 dilution. Nuclei were counterstained with Hoechst 33342 (blue, lower panel).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C1 antibody (ab192785)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue, labeling AKR1C1 with ab192785 at 7.5 μg/ml.
Protocols
References
This product has been referenced in:
- Narzt MS et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol 20:467-482 (2019). Read more (PubMed: 30466060) »
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . Read more (PubMed: 29018201) »
