Product nameAnti-AKR1C1/AKR1C2 antibody [4B6AF3]
See all AKR1C1/AKR1C2 primary antibodies
DescriptionMouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
Full length native protein (purified). This information is considered to be commercially sensitive.
- WB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 0.357% HEPES
Concentration information loading...
Purification notesPurity >90% by SDS-PAGE.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab131375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)|
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HeLa Cell Lysate at 15 µg
Lane 3 : HepG2 Cell Lysate at 15 µg
Lane 4 : Human Liver Homogenate (HLH) at 10 µg
Lane 5 : Human Heart Homogenate (HHH) at 10 µg
Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
Lane 8 : Rat Heart Homogenate (RHH) at 10 µg
All lanes : Goat anti-Mouse AP at 1/3000 dilution
Predicted band size: 37 kDa
0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody.
Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.
This product has been referenced in:
- Li C et al. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer. J Biomol Screen 21:101-7 (2016). Read more (PubMed: 26318406) »
- Connarn JN et al. Metabolism of bupropion by carbonyl reductases in liver and intestine. Drug Metab Dispos 43:1019-27 (2015). Read more (PubMed: 25904761) »