Overview

  • Product name

    Anti-AKR1C1/AKR1C2 antibody [4B6AF3]
    See all AKR1C1/AKR1C2 primary antibodies
  • Description

    Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab131375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)

Target

  • Database links

  • Alternative names

    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3-alpha-HSD3 antibody
    • AKR1C1 antibody
    • AKR1C2 antibody
    • Aldo-keto reductase family 1 member C1 antibody
    • Aldo-keto reductase family 1 member C2 antibody
    • Chlordecone reductase homolog HAKRC antibody
    • Chlordecone reductase homolog HAKRD antibody
    • DD-2 antibody
    • DD/BABP antibody
    • DD1 antibody
    • DD1/DD2 antibody
    • DD2 antibody
    • DDH antibody
    • DDH1 antibody
    • DDH2 antibody
    • Dihydrodiol dehydrogenase 1 antibody
    • Dihydrodiol dehydrogenase 1/2 antibody
    • Dihydrodiol dehydrogenase 2 antibody
    • dihydrodiol dehydrogenase isoform DD1 antibody
    • Dihydrodiol dehydrogenase/bile acid-binding protein antibody
    • HBAB antibody
    • Hepatic dihydrodial dehydrogenase antibody
    • High-affinity hepatic bile acid-binding protein antibody
    • Indanol dehydrogenase antibody
    • MBAB antibody
    • MGC8954 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type III 3-alpha-hydroxysteroid dehydrogenase antibody
    see all

Images

  • All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa Cell Lysate at 15 µg
    Lane 3 : HepG2 Cell Lysate at 15 µg
    Lane 4 : Human Liver Homogenate (HLH) at 10 µg
    Lane 5 : Human Heart Homogenate (HHH) at 10 µg
    Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
    Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
    Lane 8 : Rat Heart Homogenate (RHH) at 10 µg

    Secondary
    All lanes : Goat anti-Mouse AP at 1/3000 dilution

    Predicted band size: 37 kDa



    0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody.
  • Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.

    The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.

References

This product has been referenced in:

  • Li C  et al. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer. J Biomol Screen 21:101-7 (2016). Read more (PubMed: 26318406) »
  • Connarn JN  et al. Metabolism of bupropion by carbonyl reductases in liver and intestine. Drug Metab Dispos 43:1019-27 (2015). Read more (PubMed: 25904761) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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