Overview

  • Product name

    Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (Alexa Fluor® 488)
    See all AKR1C1/AKR1C2 primary antibodies
  • Description

    Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2 (Alexa Fluor® 488)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Full length native protein (purified) corresponding to Human AKR1C1/AKR1C2. Purified protein cocktail.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab198598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Database links

  • Alternative names

    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3-alpha-HSD3 antibody
    • AKR1C1 antibody
    • AKR1C2 antibody
    • Aldo-keto reductase family 1 member C1 antibody
    • Aldo-keto reductase family 1 member C2 antibody
    • Chlordecone reductase homolog HAKRC antibody
    • Chlordecone reductase homolog HAKRD antibody
    • DD-2 antibody
    • DD/BABP antibody
    • DD1 antibody
    • DD1/DD2 antibody
    • DD2 antibody
    • DDH antibody
    • DDH1 antibody
    • DDH2 antibody
    • Dihydrodiol dehydrogenase 1 antibody
    • Dihydrodiol dehydrogenase 1/2 antibody
    • Dihydrodiol dehydrogenase 2 antibody
    • dihydrodiol dehydrogenase isoform DD1 antibody
    • Dihydrodiol dehydrogenase/bile acid-binding protein antibody
    • HBAB antibody
    • Hepatic dihydrodial dehydrogenase antibody
    • High-affinity hepatic bile acid-binding protein antibody
    • Indanol dehydrogenase antibody
    • MBAB antibody
    • MGC8954 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type III 3-alpha-hydroxysteroid dehydrogenase antibody
    see all

Images

  • ab198598 staining AKR1C1/AKR1C2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198598 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab198598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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