Overview

  • Product name

    Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (HRP)
    See all AKR1C1/AKR1C2 primary antibodies
  • Description

    Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Full length native protein (purified) corresponding to Human AKR1C1/AKR1C2.

  • Positive control

    • IHC-P: Normal human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab198411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Database links

  • Alternative names

    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3-alpha-HSD3 antibody
    • AKR1C1 antibody
    • AKR1C2 antibody
    • Aldo-keto reductase family 1 member C1 antibody
    • Aldo-keto reductase family 1 member C2 antibody
    • Chlordecone reductase homolog HAKRC antibody
    • Chlordecone reductase homolog HAKRD antibody
    • DD-2 antibody
    • DD/BABP antibody
    • DD1 antibody
    • DD1/DD2 antibody
    • DD2 antibody
    • DDH antibody
    • DDH1 antibody
    • DDH2 antibody
    • Dihydrodiol dehydrogenase 1 antibody
    • Dihydrodiol dehydrogenase 1/2 antibody
    • Dihydrodiol dehydrogenase 2 antibody
    • dihydrodiol dehydrogenase isoform DD1 antibody
    • Dihydrodiol dehydrogenase/bile acid-binding protein antibody
    • HBAB antibody
    • Hepatic dihydrodial dehydrogenase antibody
    • High-affinity hepatic bile acid-binding protein antibody
    • Indanol dehydrogenase antibody
    • MBAB antibody
    • MGC8954 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type III 3-alpha-hydroxysteroid dehydrogenase antibody
    see all

Images

  • IHC image of AKR1C1/AKR1C2 staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198411, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab198411 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab198411.
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