Overview

  • Product name

    Anti-AKR1C1/AKR1C2 antibody
    See all AKR1C1/AKR1C2 primary antibodies
  • Description

    Rabbit polyclonal to AKR1C1/AKR1C2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 22-218 of Human AKR1C1 (NP_001344).

  • Positive control

    • WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates. IHC-P: MDA-MB-468 xenograft ICC/IF: methanol fixed SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab96087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Database links

  • Alternative names

    • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3-alpha-HSD3 antibody
    • AKR1C1 antibody
    • AKR1C2 antibody
    • Aldo-keto reductase family 1 member C1 antibody
    • Aldo-keto reductase family 1 member C2 antibody
    • Chlordecone reductase homolog HAKRC antibody
    • Chlordecone reductase homolog HAKRD antibody
    • DD-2 antibody
    • DD/BABP antibody
    • DD1 antibody
    • DD1/DD2 antibody
    • DD2 antibody
    • DDH antibody
    • DDH1 antibody
    • DDH2 antibody
    • Dihydrodiol dehydrogenase 1 antibody
    • Dihydrodiol dehydrogenase 1/2 antibody
    • Dihydrodiol dehydrogenase 2 antibody
    • dihydrodiol dehydrogenase isoform DD1 antibody
    • Dihydrodiol dehydrogenase/bile acid-binding protein antibody
    • HBAB antibody
    • Hepatic dihydrodial dehydrogenase antibody
    • High-affinity hepatic bile acid-binding protein antibody
    • Indanol dehydrogenase antibody
    • MBAB antibody
    • MGC8954 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type III 3-alpha-hydroxysteroid dehydrogenase antibody
    see all

Images

  • Anti-AKR1C1/AKR1C2 antibody (ab96087) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS-PAGE
  • Immunohistochemical analysis of AKR1C1 in paraffin embedded MDA-MB-468 xenograft, using ab96087 at a 1/500 dilution.
  • ICC/IF image of ab96087 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96087 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Jiang W  et al. Wentilactone A induces cell apoptosis by targeting AKR1C1 gene via the IGF-1R/IRS1/PI3K/AKT/Nrf2/FLIP/Caspase-3 signaling pathway in small cell lung cancer. Oncol Lett 16:6445-6457 (2018). Read more (PubMed: 30405782) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. The immunogen for ab96087 shows 97% identity with human AKR1C2, so it will likely cross-react. This has not been confirmed experimentally. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up