Recombinant Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AKR1C1/AKR1C2 antibody [EPR11542]
See all AKR1C1/AKR1C2 primary antibodies -
Description
Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2 -
Host species
Rabbit -
Specificity
AKR1C1 and AKR1C2 (Swiss Prot: Q04828/P52895) sequences are 98% identical -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: U-87 MG cells. WB: His-tagged AKR1C2 and AKR1C2 recombinant full length proteins, HepG2, U87-MG, Human fetal liver and HeLa lysates. IP: HepG2 cell lysate. Flow Cyt: U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11542 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
1/10 - 1/100. |
Target
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Database links
- SwissProt: P52895 Human
- SwissProt: Q04828 Human
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Alternative names
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
see all
Images
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All lanes : Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution
Lane 1 : His-tagged human AKR1C1 recombinant protein, full-length
Lane 2 : His-tagged human AKR1C2 recombinant protein, full-length
Lane 3 : His-tagged human AKR1C3 recombinant protein, full-length
Lane 4 : His-tagged human AKR1C4 recombinant protein, full-length
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma- astrocytoma epithelial cell) cells labelling AKR1C1/AKR1C2 with primary antibody anti-AKR1C1/AKR1C2 (ab179448) at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in U-87 MG cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1:200 dilution. The nuclear counter stain is DAPI (blue).
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AKR1C1/AKR1C2 was immunoprecipitated from U-87 MG lysates with ab179448 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab179448 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 μg
Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179448 in U-87 MG whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of permeabilized U87-MG cellslabeling AKR1C1/AKR1C2 with ab179448 at 1/10 dilution (red),compared toa rabbit IgG negative control (green).
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All lanes : Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : U87-MG lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab179448 has been referenced in 4 publications.
- Tao L et al. CD36 accelerates the progression of hepatocellular carcinoma by promoting FAs absorption. Med Oncol 39:202 (2022). PubMed: 36175596
- Ma X et al. ABX-1431 inhibits the development of endometrial adenocarcinoma and reverses progesterone resistance by targeting MGLL. Cell Death Dis 13:1067 (2022). PubMed: 36550099
- Su Z et al. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci 21:N/A (2020). PubMed: 32102467
- Murray JR et al. Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells. Chem Res Toxicol 32:2538-2551 (2019). PubMed: 31746589