Recombinant
RabMAb

Recombinant Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448)

Overview

  • Product name

    Anti-AKR1C1/AKR1C2 antibody [EPR11542]
    See all AKR1C1/AKR1C2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2
  • Host species

    Rabbit
  • Specificity

    AKR1C1 and AKR1C2 (Swiss Prot: Q04828/P52895) sequences are 98% identical
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKR1C1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q04828

  • Positive control

    • HepG2, U87-MG, Human fetal liver and HeLa lysates; U87-MG cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Database links

    • Alternative names

      • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • 20-alpha-hydroxysteroid dehydrogenase antibody
      • 3-alpha-HSD3 antibody
      • AKR1C1 antibody
      • AKR1C2 antibody
      • Aldo-keto reductase family 1 member C1 antibody
      • Aldo-keto reductase family 1 member C2 antibody
      • Chlordecone reductase homolog HAKRC antibody
      • Chlordecone reductase homolog HAKRD antibody
      • DD-2 antibody
      • DD/BABP antibody
      • DD1 antibody
      • DD1/DD2 antibody
      • DD2 antibody
      • DDH antibody
      • DDH1 antibody
      • DDH2 antibody
      • Dihydrodiol dehydrogenase 1 antibody
      • Dihydrodiol dehydrogenase 1/2 antibody
      • Dihydrodiol dehydrogenase 2 antibody
      • dihydrodiol dehydrogenase isoform DD1 antibody
      • Dihydrodiol dehydrogenase/bile acid-binding protein antibody
      • HBAB antibody
      • Hepatic dihydrodial dehydrogenase antibody
      • High-affinity hepatic bile acid-binding protein antibody
      • Indanol dehydrogenase antibody
      • MBAB antibody
      • MGC8954 antibody
      • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
      • Type III 3-alpha-hydroxysteroid dehydrogenase antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab179448 at 1/10 dilution.

    • Flow cytometric analysis of permeabilized U87-MG cells labeling AKR1C1/AKR1C2 with ab179448 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

    • All lanes : Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : U87-MG lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    References

    ab179448 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179448.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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