Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-AKR1C1/AKR1C2 antibody [EPR11542]
See all AKR1C1/AKR1C2 primary antibodies
DescriptionRabbit monoclonal [EPR11542] to AKR1C1/AKR1C2
SpecificityAKR1C1 and AKR1C2 (Swiss Prot: Q04828/P52895) sequences are 98% identical
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKR1C1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q04828
- HepG2, U87-MG, Human fetal liver and HeLa lysates; U87-MG cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab179448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab179448 at 1/10 dilution.
Flow cytometric analysis of permeabilized U87-MG cells labeling AKR1C1/AKR1C2 with ab179448 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
All lanes : Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : U87-MG lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
ab179448 has not yet been referenced specifically in any publications.