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    akr1c3-antibody-ab137546.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-AKR1C3 antibody (ab137546)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (ab137546)
  • Western blot - Anti-AKR1C3 antibody (ab137546)

Key features and details

  • Rabbit polyclonal to AKR1C3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human AKR1C3 protein (ab167984)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-AKR1C3 antibody
    See all AKR1C3 primary antibodies
  • Description

    Rabbit polyclonal to AKR1C3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-189 of Human AKR1C3.

  • Positive control

    • HeLa, HepG2 and Molt-4 whole cell lysates; Human NCI-N87 xenograft.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human AKR1C3 protein (ab167984)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
  • Tissue specificity

    Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P42330 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8644 Human
    • Omim: 603966 Human
    • SwissProt: P42330 Human
    • Unigene: 78183 Human
    • Alternative names

      • 17 beta HSD 5 antibody
      • 17 beta hydroxysteroid dehydrogenase type 5 antibody
      • 17-beta-HSD 5 antibody
      • 17-beta-hydroxysteroid dehydrogenase type 5 antibody
      • 2-dihydrobenzene-1 antibody
      • 2-diol dehydrogenase antibody
      • 20 alpha-hydroxysteroid dehydrogenase antibody
      • 20-alpha-hydroxysteroid dehydrogenase antibody
      • 3 alpha hydroxysteroid dehydrogenase type II antibody
      • 3-alpha-HSD type 2 antibody
      • 3-alpha-HSD type II antibody
      • 3-alpha-HSD type II, brain antibody
      • 3-alpha-hydroxysteroid dehydrogenase type 2 antibody
      • AK1C3_HUMAN antibody
      • AKR1 C3 antibody
      • Akr1c18 antibody
      • AKR1C3 antibody
      • Aldo keto reductase family 1 member C3 antibody
      • Aldo-keto reductase family 1 member C3 antibody
      • brain antibody
      • Chlordecone reductase antibody
      • Chlordecone reductase homolog HAKRb antibody
      • DD-3 antibody
      • DD3 antibody
      • DDH1 antibody
      • DDX antibody
      • Dihydrodiol dehydrogenase 3 antibody
      • Dihydrodiol dehydrogenase type I antibody
      • Dihydrodiol dehydrogenase X antibody
      • HA1753 antibody
      • HAKRB antibody
      • HAKRe antibody
      • hluPGFS antibody
      • HSD17B5 antibody
      • Indanol dehydrogenase antibody
      • KIAA0119 antibody
      • PGFS antibody
      • Prostaglandin F synthase antibody
      • Testosterone 17-beta-dehydrogenase 5 antibody
      • Trans-1 antibody
      • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
      • Type IIb 3 alpha hydroxysteroid dehydrogenase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (ab137546)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (ab137546)
      Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, labelling AKR1C3 with ab137546 at 1/100 dilution.
    • Western blot - Anti-AKR1C3 antibody (ab137546)
      Western blot - Anti-AKR1C3 antibody (ab137546)
      All lanes : Anti-AKR1C3 antibody (ab137546) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HepG2 whole cell lysate
      Lane 3 : Molt-4 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 37 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab137546? Please let us know so that we can cite the reference in this datasheet.

    ab137546 has been referenced in 2 publications.

    • Liu J  et al. Characterization of a highly specific monoclonal antibody against human aldo-keto reductase AKR1C3. Steroids 143:73-79 (2019). PubMed: 30639543
    • Gibson DA  et al. Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes. Sci Rep 6:19970 (2016). IF . PubMed: 26817618

    Customer reviews and Q&As

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