Overview

  • Product name

  • Description

    Rabbit polyclonal to AKR1C3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-189 of Human AKR1C3.

  • Positive control

    • HeLa, HepG2 and Molt-4 whole cell lysates; Human NCI-N87 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
  • Tissue specificity

    Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 17 beta HSD 5 antibody
    • 17 beta hydroxysteroid dehydrogenase type 5 antibody
    • 17-beta-HSD 5 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 5 antibody
    • 2-dihydrobenzene-1 antibody
    • 2-diol dehydrogenase antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3 alpha hydroxysteroid dehydrogenase type II antibody
    • 3-alpha-HSD type 2 antibody
    • 3-alpha-HSD type II antibody
    • 3-alpha-HSD type II, brain antibody
    • 3-alpha-hydroxysteroid dehydrogenase type 2 antibody
    • AK1C3_HUMAN antibody
    • AKR1 C3 antibody
    • Akr1c18 antibody
    • AKR1C3 antibody
    • Aldo keto reductase family 1 member C3 antibody
    • Aldo-keto reductase family 1 member C3 antibody
    • brain antibody
    • Chlordecone reductase antibody
    • Chlordecone reductase homolog HAKRb antibody
    • DD-3 antibody
    • DD3 antibody
    • DDH1 antibody
    • DDX antibody
    • Dihydrodiol dehydrogenase 3 antibody
    • Dihydrodiol dehydrogenase type I antibody
    • Dihydrodiol dehydrogenase X antibody
    • HA1753 antibody
    • HAKRB antibody
    • HAKRe antibody
    • hluPGFS antibody
    • HSD17B5 antibody
    • Indanol dehydrogenase antibody
    • KIAA0119 antibody
    • PGFS antibody
    • Prostaglandin F synthase antibody
    • Testosterone 17-beta-dehydrogenase 5 antibody
    • Trans-1 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type IIb 3 alpha hydroxysteroid dehydrogenase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, labelling AKR1C3 with ab137546 at 1/100 dilution.
  • All lanes : Anti-AKR1C3 antibody (ab137546) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Liu J  et al. Characterization of a highly specific monoclonal antibody against human aldo-keto reductase AKR1C3. Steroids 143:73-79 (2019). Read more (PubMed: 30639543) »
  • Gibson DA  et al. Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes. Sci Rep 6:19970 (2016). IF . Read more (PubMed: 26817618) »
See all 2 Publications for this product

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