Key features and details
- Rabbit polyclonal to AKR1C3
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-AKR1C3 antibody
See all AKR1C3 primary antibodies
DescriptionRabbit polyclonal to AKR1C3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant full length protein corresponding to Human AKR1C3.
Database link: P42330
- K562, Jurkat, HepG2, A549 and SW620 cell extracts; Human esophageal cancer, rat kidney and rat brain tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 37 kDa.|
FunctionCatalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Tissue specificityExpressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
- 17-beta-HSD 5 antibody
All lanes : Anti-AKR1C3 antibody (ab196673) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : Jurkat cell extract
Lane 3 : HepG2 cell extract
Lane 4 : A549 cell extract
Lane 5 : SW620 cell extract
Predicted band size: 37 kDa
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling AKR1C3 with ab196673 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling AKR1C3 with ab196673 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling AKR1C3 with ab196673 at 1/100 dilution.
ab196673 has not yet been referenced specifically in any publications.