Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab27491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).|
FunctionCatalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Tissue specificityExpressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
- 17-beta-HSD 5 antibody
Anti-AKR1C3 antibody (ab27491) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
This product has been referenced in:
- Phillips RJ et al. Prostaglandin pathway gene expression in human placenta, amnion and choriodecidua is differentially affected by preterm and term labour and by uterine inflammation. BMC Pregnancy Childbirth 14:241 (2014). IHC . Read more (PubMed: 25048443) »
- Cornel KM et al. Overexpression of 17ß-hydroxysteroid dehydrogenase type 1 increases the exposure of endometrial cancer to 17ß-estradiol. J Clin Endocrinol Metab 97:E591-601 (2012). Read more (PubMed: 22362820) »