Key features and details
- Rabbit polyclonal to AKR1C3 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AKR1C3 antibody - C-terminal
See all AKR1C3 primary antibodies
DescriptionRabbit polyclonal to AKR1C3 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within C terminal amino acids 261-323 of Human AKR1C3.
- HeLa cells; HepG2 whole cell lysate (ab7900); Human NCI-N87 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Tissue specificityExpressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
- 17-beta-HSD 5 antibody
Anti-AKR1C3 antibody - C-terminal (ab137545) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling AKR1C3 with ab137545 at 1/200 dilution. Lower panel is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labelling AKR1C3 with ab137545 at 1/100 dilution.
ab137545 has not yet been referenced specifically in any publications.