Overview

  • Product name

    Anti-AKR1C3 antibody [EPR16725]
    See all AKR1C3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16725] to AKR1C3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AKR1C3 aa 1-100. The exact sequence is proprietary.
    Database link: P42330

  • Positive control

    • WB: Human fetal brain, fetal kidney and fetal liver lysates; MOLT-4 cell lysate. IP: Human fetal liver whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP 1/50.

Target

  • Function

    Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
  • Tissue specificity

    Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 17 beta HSD 5 antibody
    • 17 beta hydroxysteroid dehydrogenase type 5 antibody
    • 17-beta-HSD 5 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 5 antibody
    • 2-dihydrobenzene-1 antibody
    • 2-diol dehydrogenase antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3 alpha hydroxysteroid dehydrogenase type II antibody
    • 3-alpha-HSD type 2 antibody
    • 3-alpha-HSD type II antibody
    • 3-alpha-HSD type II, brain antibody
    • 3-alpha-hydroxysteroid dehydrogenase type 2 antibody
    • AK1C3_HUMAN antibody
    • AKR1 C3 antibody
    • Akr1c18 antibody
    • AKR1C3 antibody
    • Aldo keto reductase family 1 member C3 antibody
    • Aldo-keto reductase family 1 member C3 antibody
    • brain antibody
    • Chlordecone reductase antibody
    • Chlordecone reductase homolog HAKRb antibody
    • DD-3 antibody
    • DD3 antibody
    • DDH1 antibody
    • DDX antibody
    • Dihydrodiol dehydrogenase 3 antibody
    • Dihydrodiol dehydrogenase type I antibody
    • Dihydrodiol dehydrogenase X antibody
    • HA1753 antibody
    • HAKRB antibody
    • HAKRe antibody
    • hluPGFS antibody
    • HSD17B5 antibody
    • Indanol dehydrogenase antibody
    • KIAA0119 antibody
    • PGFS antibody
    • Prostaglandin F synthase antibody
    • Testosterone 17-beta-dehydrogenase 5 antibody
    • Trans-1 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type IIb 3 alpha hydroxysteroid dehydrogenase antibody
    see all

Images

  • Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.

  • AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Human fetal liver whole cell lysate 10ug (Input). Lane 2: ab203834 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

References

ab203834 has not yet been referenced specifically in any publications.

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