Recombinant Anti-AKR1C3 antibody [EPR16725] (ab203834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16725] to AKR1C3
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-AKR1C3 antibody [EPR16725]
See all AKR1C3 primary antibodies -
Description
Rabbit monoclonal [EPR16725] to AKR1C3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AKR1C3 aa 1-100. The exact sequence is proprietary.
Database link: P42330 -
Positive control
- WB: Human fetal brain, fetal kidney and fetal liver lysates; MOLT-4 cell lysate. IP: Human fetal liver whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16725 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). | |
| IP | 1/50. |
Target
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Function
Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. -
Tissue specificity
Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8644 Human
- Omim: 603966 Human
- SwissProt: P42330 Human
- Unigene: 78183 Human
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Alternative names
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
- 17-beta-HSD 5 antibody
see all
Images
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![Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)](https://www.abcam.com/ps/products/203/ab203834/Images/ab203834-248148-anti-akr1c3-antibody-epr16725-western-blot.jpg)
Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/5000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)](https://www.abcam.com/ps/products/203/ab203834/Images/ab203834-248147-anti-akr1c3-antibody-epr16725-western-blot.jpg)
Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)](https://www.abcam.com/ps/products/203/ab203834/Images/ab203834-248146-anti-akr1c3-antibody-epr16725-western-blot.jpg)
Western blot - Anti-AKR1C3 antibody [EPR16725] (ab203834)All lanes : Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.
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![Immunoprecipitation - Anti-AKR1C3 antibody [EPR16725] (ab203834)](https://www.abcam.com/ps/products/203/ab203834/Images/ab203834-248145-anti-akr1c3-antibody-epr16725-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AKR1C3 antibody [EPR16725] (ab203834)AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Human fetal liver whole cell lysate 10ug (Input). Lane 2: ab203834 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Anti-AKR1C3 antibody [EPR16725] (ab203834)](https://www.abcam.com/ps/products/203/ab203834/Images/ab203834-6-benefits-of-recombinant-antibodies.png)
Anti-AKR1C3 antibody [EPR16725] (ab203834)
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab203834 has been referenced in 1 publication.
- Zhou M et al. A Mansonone Derivative Coupled with Monoclonal Antibody 4D5-Modified Chitosan Inhibit AKR1C3 to Treat Castration-Resistant Prostate Cancer. Int J Nanomedicine 15:3087-3098 (2020). PubMed: 32431503
