Overview

  • Product name

    Anti-AKR1C3 antibody [EPR16726]
    See all AKR1C3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16726] to AKR1C3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AKR1C3 aa 200-300. The exact sequence is proprietary.
    Database link: P42330

  • Positive control

    • WB: Human fetal kidney lysate; MOLT-4, A549 and HepG2 whole cell lysates, ICC/IF: HepG2 and A549 cells. Flow Cyt: A549 cells. IP: HepG2 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF 1/100.
Flow Cyt 1/70.
IP 1/30.

Target

  • Function

    Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
  • Tissue specificity

    Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 17 beta HSD 5 antibody
    • 17 beta hydroxysteroid dehydrogenase type 5 antibody
    • 17-beta-HSD 5 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 5 antibody
    • 2-dihydrobenzene-1 antibody
    • 2-diol dehydrogenase antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-hydroxysteroid dehydrogenase antibody
    • 3 alpha hydroxysteroid dehydrogenase type II antibody
    • 3-alpha-HSD type 2 antibody
    • 3-alpha-HSD type II antibody
    • 3-alpha-HSD type II, brain antibody
    • 3-alpha-hydroxysteroid dehydrogenase type 2 antibody
    • AK1C3_HUMAN antibody
    • AKR1 C3 antibody
    • Akr1c18 antibody
    • AKR1C3 antibody
    • Aldo keto reductase family 1 member C3 antibody
    • Aldo-keto reductase family 1 member C3 antibody
    • brain antibody
    • Chlordecone reductase antibody
    • Chlordecone reductase homolog HAKRb antibody
    • DD-3 antibody
    • DD3 antibody
    • DDH1 antibody
    • DDX antibody
    • Dihydrodiol dehydrogenase 3 antibody
    • Dihydrodiol dehydrogenase type I antibody
    • Dihydrodiol dehydrogenase X antibody
    • HA1753 antibody
    • HAKRB antibody
    • HAKRe antibody
    • hluPGFS antibody
    • HSD17B5 antibody
    • Indanol dehydrogenase antibody
    • KIAA0119 antibody
    • PGFS antibody
    • Prostaglandin F synthase antibody
    • Testosterone 17-beta-dehydrogenase 5 antibody
    • Trans-1 antibody
    • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody
    • Type IIb 3 alpha hydroxysteroid dehydrogenase antibody
    see all

Images

  • Lanes 1-2 : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/1000 dilution
    Lane 3 : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3: 2 seconds; Lane 2: 30 seconds.

  • All lanes : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control)
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with AKR1C3 peptide on the membrane
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with AKR1C4 peptide on the membrane

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at  1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at  1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on A549 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at  1/1000 dilution and Goat Anti-Mouse IgG (Alexa Fluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • AKR1C3 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab209899 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209899 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab209899 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab209899 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab209899 has not yet been referenced specifically in any publications.

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